[罗通胶囊的高效液相色谱指纹图谱分析]
[Fingerprint analysis of Luotong capsule by HPLC].
作者信息
Zhao Xiao-li, Di Liu-qing, Wu Hao, Li Wei-dong
机构信息
Institue of TCM, Nanjing University of Traitional Chinese Medicine, Nanjing 210029, China.
出版信息
Zhong Yao Cai. 2007 Apr;30(4):460-3.
OBJECTIVE
To develop the chromatographic fingerprints of Luotong capsule by HPLC.
METHODS
The separation was performed on YMC-PacK ODS (4.6 mm x 250 mm, 5 microm) column with a mobile phase consisting of acetonitrile -0.5% phosphoric acid as gradient elution at the flow rate of 1.0 ml/min. The detective wavelength was 276 nm. The column temperature was 30 degrees C.
RESULTS
A standard HPLC fingerprint procedure was developed for Luotong capsule with 18 common peaks. The similarity of 10 batches of Luotong capsule was not lower than 0.978.
CONCLUSION
The mehtod is accurate, repeatable and useful for the quality control of Luotong capsule.
目的
采用高效液相色谱法建立络通胶囊的色谱指纹图谱。
方法
采用YMC-PacK ODS柱（4.6 mm×250 mm，5μm），以乙腈-0.5%磷酸为流动相进行梯度洗脱，流速为1.0 ml/min。检测波长为276 nm。柱温为30℃。
结果
建立了络通胶囊的标准高效液相色谱指纹图谱，共有18个共有峰。10批络通胶囊的相似度均不低于0.978。
结论
该方法准确、可重复，可用于络通胶囊的质量控制。
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