[Optimize extraction process of polysaccharide in Trillium tschonoskii].

OBJECTIVE

To optimize the extraction process of polysaccharide in Trillium tschonoskii.

METHODS

The influence of temperature, time, solid-liquid ratio and extraction times on extraction yield of the polysaccharide in fleshy roots of Trillium tschonokii were discussed with orthogonal test method.

RESULTS

The impact sequence of the factors on the extraction rate of polysaccharide in Trillium tschonoskii was as follows: extraction times > time > solid-liquid ratio > temperature; The optimal extraction condition was extraction temperature of 80 degrees C, extraction time of 4.5h, solid-liquid ratio of 1:40 and extracted three times.

CONCLUSION

Under these optimal conditions, the extracting rates of polysaccharide in Trillium tschonoskii is 6.75%. The content of polysaccharide is high which can be exploited and utilized as another new ingredient.