用于等电聚焦的载体两性电解质分级分离

Fractionation of carrier ampholytes for isoelectric focusing.

作者信息

Gianazza E, Pagani M, Luzzana M, Righetti P G

出版信息

J Chromatogr. 1975 Jun 18;109(2):357-64. doi: 10.1016/s0021-9673(01)91808-4.

Abstract

A simple method for fractionating synthetic carrier ampholytes is reported, based on the principle of continuous-flow isoelectric focusing in gel-stabilized layers. An 8% ampholyte solution, encompassing the pH range 3-9.5, is separated into 12 fractions in a chamber filled with Sephadex G-100 by a continuous-flow technique. We are thus able to obtain ampholytes of narrow pH range, encompassing approximately 2 pH units, whose resolving power is comparable with that obtained with commercial Ampholine covering similar pH ranges.

摘要

报道了一种基于凝胶稳定层中连续流动等电聚焦原理对合成载体两性电解质进行分级分离的简单方法。一种涵盖pH范围3 - 9.5的8%两性电解质溶液，通过连续流动技术在装有葡聚糖G - 100的腔室中被分离成12个级分。因此，我们能够获得pH范围狭窄（约2个pH单位）的两性电解质，其分辨能力与覆盖类似pH范围的市售两性电解质相当。