从宽范围两性电解质制剂中分离出的窄范围两性电解质的性质。
Properties of narrow-range ampholytes isolated from wide-range ampholyte preparations.
作者信息
Binion S B, Rodkey L S, Egen N B, Bier M
出版信息
Anal Biochem. 1983 Jan;128(1):71-6. doi: 10.1016/0003-2697(83)90346-9.
A simple procedure for obtaining useful narrow-pH-range ampholytes from inexpensive laboratory-synthesized ampholytes by preparative isoelectric focusing in Pevikon is described. The narrow-range ampholytes prepared in this way are comparable to commercial ampholyte preparations as judged by conductivity, buffer capacity, pH gradient formation, and resolving power. These inexpensive narrow-range ampholytes are particularly well suited to preparative isoelectric focusing applications requiring large quantities of ampholytes.
本文描述了一种简单的方法，可通过在Pevikon中进行制备性等电聚焦，从廉价的实验室合成两性电解质中获得有用的窄pH范围两性电解质。通过电导率、缓冲容量、pH梯度形成和分辨能力判断，以这种方式制备的窄范围两性电解质与市售两性电解质制剂相当。这些廉价的窄范围两性电解质特别适用于需要大量两性电解质的制备性等电聚焦应用。
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