Baudys M, Meloun B, Gan-Erdene T, Pohl J, Kostka V
Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, Prague, CSFR.
Biol Chem Hoppe Seyler. 1990 Jun;371(6):485-91. doi: 10.1515/bchm3.1990.371.1.485.
Bovine spleen cathepsin B contains 7 disulfide bridges. Cleavage of the enzyme with cyanogen bromide gives rise to a large and a small fragment. The former contains all disulfide bridges. Their arrangement was determined by analysis of amino-acid sequences and compositions of subfragments prepared by cleavage of the large cyanogen-bromide fragment with trypsin, chymotrypsin and the staphylococcal proteinase using specific methods for the detection of S-S-bonds. Disulfide bridges link together Cys14-Cys43, Cys26-Cys71, Cys62-Cys128, Cys63-Cys67, Cys100-Cys132, Cys108-Cys119 and Cys148-Cys252.
牛脾组织蛋白酶B含有7个二硫键。用溴化氰裂解该酶会产生一个大片段和一个小片段。前者包含所有二硫键。通过使用检测S-S键的特定方法，分析用胰蛋白酶、胰凝乳蛋白酶和葡萄球菌蛋白酶裂解溴化氰大片段所制备的亚片段的氨基酸序列和组成，确定了它们的排列方式。二硫键将Cys14与Cys43、Cys26与Cys71、Cys62与Cys128、Cys63与Cys67、Cys100与Cys132、Cys108与Cys119以及Cys148与Cys252连接在一起。
