Miyake Nahoko, Sato Toru, Maki Yoshinobu
Division of Prosthodontics, Department of Clinical Oral Health Science, Tokyo Dental College, 2-9-18 Misaki-cho, Chiyoda-ku, Tokyo 101-0061, Japan.
Bull Tokyo Dent Coll. 2013;54(2):97-101. doi: 10.2209/tdcpublication.54.97.
The aim of the present study was to examine the adsorption of bovine serum albumin (BSA) to hydroxyapatite surfaces by means of zeta potential. The electrophoretic mobility of both hydroxyapatite and BSA were negative, with BSA itself less negative than hydroxyapatite. The zeta potential of the surface of BSA-adsorbed hydroxyapatite was significantly more negative than that of hydroxyapatite alone (p<0.0001). The BSA histogram indicated two negative peaks, and the zeta potential of BSA-adsorbed hydroxyapatite also showed two similar negative peaks. These results suggest that BSA adsorption to hydroxyapatite surfaces is related to electrostatic interaction.
本研究的目的是通过ζ电位检测牛血清白蛋白（BSA）在羟基磷灰石表面的吸附情况。羟基磷灰石和BSA的电泳迁移率均为负，其中BSA本身的负性比羟基磷灰石小。吸附了BSA的羟基磷灰石表面的ζ电位明显比单独的羟基磷灰石更负（p<0.0001）。BSA的直方图显示有两个负峰，吸附了BSA的羟基磷灰石的ζ电位也显示出两个类似的负峰。这些结果表明，BSA在羟基磷灰石表面的吸附与静电相互作用有关。
