Biochemistry Laboratory, Department of Crop Improvement, College of Agriculture, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur, HP, 176062, India,
Appl Biochem Biotechnol. 2013 Oct;171(4):847-73. doi: 10.1007/s12010-013-0399-4. Epub 2013 Aug 1.
The research on the RNA interference (RNAi) for the control of insect pests has made significant growth in recent years. The availability of the genomic sequences of insects has further widened the horizons for the testing of this technology to various insect groups. Different modes of application of double-stranded RNA (dsRNA) have been tested; however, the practicability of delivery of dsRNA in insects still remains the biggest challenge. Till date, the oral delivery of dsRNA in insects is one of the efficient approaches for the practical application of this technique. The uptake of dsRNA from the insect gut is mediated either by SID-1/SID-2 transmembrane proteins or by endocytosis; however, the systemic RNAi machinery still remains to be revealed in insect species. The RNAi-mediated gene knockdown has shown striking results in different insect groups, pointing it to be the upcoming technique for insect control. However, before the successful application of this technique for insect control, some potential issues need to be resolved. This review presents the account of prospects and challenges for the use of this technology for insect control.
近年来，RNA 干扰（RNAi）在害虫防治方面的研究取得了显著进展。昆虫基因组序列的可用性进一步拓宽了该技术在各种昆虫群体中的测试范围。已经测试了双链 RNA（dsRNA）的不同应用模式，但 dsRNA 在昆虫体内的传递实用性仍然是最大的挑战。迄今为止，dsRNA 在昆虫体内的口服递送是该技术实际应用的有效方法之一。dsRNA 从昆虫肠道中的摄取是通过 SID-1/SID-2 跨膜蛋白或内吞作用介导的；然而，昆虫物种中的系统 RNAi 机制仍有待揭示。RNAi 介导的基因敲低在不同的昆虫群体中显示出显著的结果，表明它将成为昆虫控制的下一个技术。然而，在成功将该技术应用于昆虫控制之前，需要解决一些潜在问题。本文综述了该技术在昆虫控制中的应用前景和挑战。
