Li Jie, Wang Xiao-Ping, Wang Man-Qun, Ma Wei-Hua, Hua Hong-Xia
Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, Huazhong Agricultural University, Wuhan, China.
Insect Sci. 2013 Feb;20(1):31-9. doi: 10.1111/j.1744-7917.2012.01550.x. Epub 2012 Oct 5.
RNA interference (RNAi) suppresses the expression of target genes by post-transcriptional regulation. Because double-stranded RNA (dsRNA) mediated gene silencing is a conserved mechanism in many eukaryotes, RNAi has become a valuable tool for unveiling gene function in many model insects. Recent research has also shown that RNAi can also be effective in the downregulation of target genes in Hemiptera. In this review, we discuss the use of the RNAi technique in gene functional analysis in hemipterans, highlighting the methods of dsRNA uptake by these insects and discuss the knock-down efficiency of these techniques. Although the RNAi technique has disadvantages, our primary goal here is to determine whether it can be exploited further in the discovery of new gene functions, and as a pest control strategy, in some important Hemipteran pests.
RNA干扰（RNAi）通过转录后调控抑制靶基因的表达。由于双链RNA（dsRNA）介导的基因沉默是许多真核生物中的一种保守机制，RNAi已成为揭示许多模式昆虫基因功能的重要工具。最近的研究还表明，RNAi在半翅目昆虫中对靶基因的下调也有效。在本综述中，我们讨论了RNAi技术在半翅目昆虫基因功能分析中的应用，重点介绍了这些昆虫摄取dsRNA的方法，并讨论了这些技术的敲低效率。尽管RNAi技术存在缺点，但我们在此的主要目标是确定它是否可以在发现新基因功能方面进一步得到利用，并作为一种害虫防治策略应用于一些重要的半翅目害虫。
