Booth R F, Honey A C, Martin J F, Lindon J C, Farrant R D, Carpenter T A, Hall L D
Department of Physical Sciences, Wellcome Research Laboratories, Beckenham, Kent, UK.
NMR Biomed. 1990 Apr;3(2):95-100. doi: 10.1002/nbm.1940030209.
High resolution 1H and 13C NMR spectroscopic measurements including 1H/13C 2D correlation and magnetic resonance imaging (MRI) studies, have been carried out on intact rabbit aortic tissues ex vivo using animals fed both normal and high cholesterol diets. The results show that 1H and 13C NMR spectroscopy can distinguish mobile lipids and can differentiate between normal triglyceride content and cholesterol-enriched lipids, in intact tissue, There were considerable differences in the level of deposition of cholesteryl esters in animals all fed on the same diet. Confirmation is presented of temperature-dependent differences in mobility and organization between the triglycerides found in control tissue and the cholesteryl esters found in aortas from high lipid diet animals. Water-suppressed MRI showed evidence of lipid accumulation in the aortas of high cholesterol diet rabbits. It is concluded that the hypercholesterolaemic rabbit model of atherosclerosis, coupled with such NMR methods, may offer a noninvasive method of monitoring disease development, allowing the evaluation of the effect of therapeutic agents on the progress of atherosclerosis.
利用喂食正常饮食和高胆固醇饮食的动物，对离体的完整兔主动脉组织进行了高分辨率1H和13C核磁共振波谱测量，包括1H/13C二维相关和磁共振成像（MRI）研究。结果表明，1H和13C核磁共振波谱可以区分可移动脂质，并能在完整组织中区分正常甘油三酯含量和富含胆固醇的脂质。在喂食相同饮食的动物中，胆固醇酯的沉积水平存在显著差异。证实了对照组织中发现的甘油三酯与高脂饮食动物主动脉中发现的胆固醇酯在流动性和组织性方面存在温度依赖性差异。水抑制MRI显示高胆固醇饮食兔主动脉中有脂质积累的证据。得出的结论是，动脉粥样硬化的高胆固醇血症兔模型，结合此类核磁共振方法，可能提供一种监测疾病发展的非侵入性方法，从而能够评估治疗药物对动脉粥样硬化进展的影响。
