Duan Jing-Bo, Liu Wen-Qing, Zhang Yu-Jun, Zhao Nan-Jing, Wang Zhi-Gang, Yin Gao-Fang, Fang Li, Liu Jing
Key Laboratory of Environmental Optics and Technology, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei 230031, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2013 May;33(5):1262-5.
Spectrofluorometry of chlorella pyrenoidosa was studied by three dimensional excitation-emission (3DEEM) fluorescence spectroscopy and synchronous scan fluorescence spectroscopy with Delta gamma = 20 nm in the stress of Hg+, Cd2+, Cu2+ and Zn2+. The conclusion from two kinds of Spectrofluorometry was the same: after 96h stress by heavy metals, the maximum fluorescence values reduced obviously, chlorophyll-a and chlorophyll-b in the photosynthetic system were seriously damaged by heavy metal. Further analysis of the correlations between heavy metal concentration and fluorescence quenching efficiency I0/I can conclude that the toxicity of heavy metal and the fluorescence quenching efficiency I0/I were positively correlated, and they all increased with the heavy metal concentration and stress time. For one kind of heavy metals, synchronous scan fluorometry is a sensitive method for its toxicity assessment. Compared to 3DEEM fluorescence spectroscopy, synchronous scan fluorescence spectroscopy is less time consuming and of higher selectivity. It is suitable to assess the toxicity of pollutions in water.
利用三维激发-发射（3DEEM）荧光光谱法和同步扫描荧光光谱法（Δγ = 20 nm），研究了在Hg+、Cd2+、Cu2+和Zn2+胁迫下小球藻的荧光光谱。两种荧光光谱法得出的结论一致：在重金属胁迫96小时后，最大荧光值明显降低，光合系统中的叶绿素a和叶绿素b受到重金属的严重破坏。进一步分析重金属浓度与荧光猝灭效率I0/I之间的相关性可以得出，重金属的毒性与荧光猝灭效率I0/I呈正相关，且二者均随重金属浓度和胁迫时间的增加而增加。对于一种重金属而言，同步扫描荧光光谱法是一种评估其毒性的灵敏方法。与3DEEM荧光光谱法相比，同步扫描荧光光谱法耗时更少且选择性更高。它适用于评估水中污染物的毒性。
