Liu Hong-Wei, Xie Hua-Lin, Nie Xi-Du
College of Material and Chemical Engineering, Hunan Institute of Technology, Hengyang 421002, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2013 May;33(5):1354-6.
Samples of bitter almond were digested by microwave digestion, and trace elements amounts of B, Na, Mg, Al, P, K, Ca, Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, As, Se, Rb, Sr, Mo, Cd, Ba and Pb in sample solutions were determined by inductively coupled plasma mass spectrometry (ICP-MS). HNO3 + H2O2 was used to achieve the complete decomposition of the organic matrix in a closed-vessel microwave oven. The working parameters of the instrument were optimized. The result showed that the relative standard deviation (RSD) was less than 4.79% for all the elements, and the recovery was 90.00%-109.30% by adding standard recovery experiment. This method was simple, sensitive and precise and can perform simultaneo multi-elements determination for bitter almond, which could satisfy the sample examination request and provide scientific rationale for determining inorganic elements in bitter almond.
采用微波消解对苦杏仁样品进行消解，然后用电感耦合等离子体质谱法（ICP-MS）测定样品溶液中硼（B）、钠（Na）、镁（Mg）、铝（Al）、磷（P）、钾（K）、钙（Ca）、钛（Ti）、钒（V）、铬（Cr）、锰（Mn）、铁（Fe）、钴（Co）、镍（Ni）、铜（Cu）、锌（Zn）、砷（As）、硒（Se）、铷（Rb）、锶（Sr）、钼（Mo）、镉（Cd）、钡（Ba）和铅（Pb）等微量元素的含量。在密闭微波消解仪中，使用硝酸（HNO₃）+过氧化氢（H₂O₂）实现有机基体的完全分解。对仪器的工作参数进行了优化。结果表明，所有元素的相对标准偏差（RSD）均小于4.79%，加标回收实验的回收率为90.00% - 109.30%。该方法简便、灵敏、准确，可同时对苦杏仁进行多元素测定，能够满足样品检测要求，为苦杏仁中无机元素的测定提供科学依据。
