Zhang Zhen-Nian, Li Ji-Ying, Zhao Yang, Wang Jing-Qing, Huang Chi, Fan Gang-qi
Department of Cerebral Diseases, Third Affiliated Hospital of Nanjing University of Tradi tional Chinese Medicine, Nanjing 210001, China.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2013 May;33(5):646-50.
To evaluate effects of Tongnao Huoluo acupuncture therapy (THAT) on Bcl-2 and Caspase-3 rats with acute cerebral infarction (ACI).
Totally 264 SD rats were randomly divided into 5 groups, i.e. the THAT group (n =72), the thrombolysis group (n =72), the body acupuncture group (n =72), the ischemia control group (n =24), and the sham-operation group (n =24). Successfully modeled rats were recruited in all groups except the sham-operation group. Rats in the THAT group, the thrombolysis group, and the body acupuncture group were divided into 3 subgroups according to the disease occurrence time, i.e., < or = 1.5 h THAT group, 1.5+ -2 h THAT group, and 2+ -3 h THAT group. The neuroethological scores were assessed at 6, 24, and 72 h after treatment. The expressions of Bcl-2 and Caspase-3 were detected using immunohistochemical staining at 24 and 72 h respectively.
In aspect of improving scores of neurological functions: At 6 h after treatment within 2 h after the disease occurrence, the neuroethological scores were lowered more obviously in the thrombolysis group than in the THAT group (P <0.05). There was statistical difference at 24 and 72 h within 2 - 3 h after the,disease occurrence between the THAT group and the thrombolysis group (P <0.05). Compared with before treatment, there was statistical difference at 24 and 72 h within 3 h after the disease occurrence (P <0. 05, P <0.01). In aspect of lowering the expression of Caspase-3 and elevating the expression of Bcl-2: There was statistical difference in lowering the expression of Caspase-3 and elevating the expression of Bcl-2 between the THAT group and the thrombolysis group at 72 h within 2 -3 after the disease occurrence (P <0.05, P < 0.01).
THAT showed favorable effects in lowering neuroethological scores, lowering expression of Caspase-3, and elevating the expression of Bcl-2 of ACI rats.
评价通脑活络针刺疗法（THAT）对急性脑梗死（ACI）大鼠Bcl-2和Caspase-3的影响。
将264只SD大鼠随机分为5组，即通脑活络针刺疗法组（n =72）、溶栓组（n =72）、体针组（n =72）、缺血对照组（n =24）和假手术组（n =24）。除假手术组外，其余各组均成功造模。通脑活络针刺疗法组、溶栓组和体针组大鼠根据发病时间分为3个亚组，即发病时间≤1.5 h通脑活络针刺疗法组、1.5+～2 h通脑活络针刺疗法组和2+～3 h通脑活络针刺疗法组。治疗后6、24和72 h进行神经行为学评分。分别于24和72 h采用免疫组织化学染色法检测Bcl-2和Caspase-3的表达。
在改善神经功能评分方面：发病后2 h内治疗6 h时，溶栓组神经行为学评分降低较通脑活络针刺疗法组更明显（P <0.05）。发病后2～3 h内24和72 h时，通脑活络针刺疗法组与溶栓组比较差异有统计学意义（P <0.05）。与治疗前比较，发病后3 h内24和72 h时差异有统计学意义（P <0.05，P <0.01）。在降低Caspase-3表达和升高Bcl-2表达方面：发病后2～3 h内72 h时，通脑活络针刺疗法组与溶栓组在降低Caspase-3表达和升高Bcl-2表达方面差异有统计学意义（P <0.05，P <0.01）。
通脑活络针刺疗法在降低ACI大鼠神经行为学评分、降低Caspase-3表达和升高Bcl-2表达方面显示出良好效果。
