Chen Feng, Yan Zhi-kang, Yang Bo
Department of Acu-moxibustion, People's Hospital, Affiliated to Wuhan University, Wuhan 430060, China.
Zhen Ci Yan Jiu. 2009 Dec;34(6):363-7.
To investigate the underlying mechanism of electroacupuncture (EA) in anti-apoptosis effects in cerebral ischemia-reperfusion injury (CI/RI) rats.
Forty-eight SD rats were randomized into sham-operation (sham), model and acupuncture groups with 16 cases in each. CI/RI model was established by occlusion of the middle cerebral artery for 2 h and reperfusion for 24 h. Neuronal apoptosis and expression levels of the cerebral bcl-2 and caspase-3 after administration of EA (MS 6, MS 7) were detected by immunohistochemistry. Whole blood viscosity, erythrocyte deformability and erythrocyte agreeability in rats after local CI/RI were assayed by using Automatic Blood Rheometer.
In comparison with control group, bcl-2 and caspase-3 immunoactivity of the ischemic cerebral tissue, high, medium and low shear rates, aggregation index and rigidity index of the erythrocytes in model group increased significantly (P<0.05), while the erythrocyte deformity index reduced strikingly (P<0.05). Compared with model group, bcl-2 immunoactivity and erythrocyte deformity of acupuncture group increased remarkably (P<0.05), and caspase-3 immunoactivity, high, medium and low shear rates of the whole blood viscosity, and erythrocyte aggregation index and rigidity index decreased considerably after acupuncture (P<0.05), suggesting an improvement of the erythrocyte deformability and agreeability after acupuncture.
Acupuncture can resist neuronal apoptosis during CI/RI, which may be closely associated with its effects in upregulating bcl-2 expression, downregulating caspase-3 expression. Moreover, acupuncture can significantly improve the erythrocyte deformability and reduce blood viscosity and erythrocyte agreeability.
探讨电针（EA）对脑缺血再灌注损伤（CI/RI）大鼠抗凋亡作用的潜在机制。
48只SD大鼠随机分为假手术组（sham）、模型组和针刺组，每组16只。采用大脑中动脉闭塞2小时再灌注24小时的方法建立CI/RI模型。通过免疫组化检测电针（MS 6、MS 7）给药后脑组织神经元凋亡及bcl-2和caspase-3的表达水平。使用自动血液流变仪检测局部CI/RI大鼠的全血黏度、红细胞变形性和红细胞聚集性。
与对照组相比，模型组缺血脑组织的bcl-2和caspase-3免疫活性、红细胞高、中、低切变率、聚集指数和刚性指数显著升高（P<0.05），而红细胞变形指数显著降低（P<0.05）。与模型组相比，针刺组的bcl-2免疫活性和红细胞变形性显著升高（P<0.05），针刺后caspase-3免疫活性、全血黏度高、中、低切变率以及红细胞聚集指数和刚性指数显著降低（P<0.05），提示针刺后红细胞变形性和聚集性得到改善。
针刺可抵抗CI/RI期间的神经元凋亡，这可能与其上调bcl-2表达、下调caspase-3表达的作用密切相关。此外，针刺可显著改善红细胞变形性，降低血液黏度和红细胞聚集性。
