Luppi A, Bonilauri P, Dottori M, Gherpelli Y, Biasi G, Merialdi G, Maioli G, Martelli P
Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSLER), Brescia, Italy.
Transbound Emerg Dis. 2015 Feb;62(1):67-71. doi: 10.1111/tbed.12081. Epub 2013 Mar 21.
Four-hundred and forty-two F4+ pathogenic Escherichia coli were isolated in a period of 10 years (2002-2011), from pigs that were suffering from diarrhoea belonging to Italian swine herds. The strains were analysed for their susceptibility to 12 antimicrobials using the disc diffusion method. During the study period, a statistically significant proportion of isolates resistant to enrofloxacin (14.5-89.3%), marbofloxacin (5.4-60.7%), flumequine (49.1-92.9%), danofloxacin (21.6-80%), florfenicol (9.8-64.3%), thiamphenicol (50-92%) and cefquinome (3.8-44%) was recorded. An increase in resistance (not statistically significant) to gentamicin (63.6-85.7%), apramycin (61.8-82.1%), trimethoprim-sulphamethoxazole (75-89.3%), tetracycline (97-100%) and erythromycin (92.4-100%) was also observed. Based on antimicrobial multiresistance, the strains were collected into three groups: I. resistant to 2-5 antimicrobials; II. resistant to 6-8 antimicrobials; III. resistant to 9-12 antimicrobials. The number of isolates belonging to the first group showed a statistically significant decrease (P < 0.05; R(2) = 0.896; r = -0.9608), while the isolates belonging to the second and third groups showed a statistically significant increase in resistance (P < 0.05; R(2) = 0.753; r = 0.8890 and P < 0.05; R(2) = 0.727; r = 0.8701, respectively) over the period of study. The results of this study suggest the need for continued monitoring of the development of resistance.
在10年期间（2002 - 2011年），从患有腹泻的意大利猪群中分离出442株F4 +致病性大肠杆菌。使用纸片扩散法分析了这些菌株对12种抗菌药物的敏感性。在研究期间，记录到对恩诺沙星（14.5 - 89.3%）、马波沙星（5.4 - 60.7%）、氟甲喹（49.1 - 92.9%）、达氟沙星（21.6 - 80%）、氟苯尼考（9.8 - 64.3%）、甲砜霉素（50 - 92%）和头孢喹肟（3.8 - 44%）耐药的分离株比例具有统计学意义。还观察到对庆大霉素（63.6 - 85.7%）、阿普拉霉素（61.8 - 82.1%）、甲氧苄啶 - 磺胺甲恶唑（75 - 89.3%）、四环素（97 - 100%）和红霉素（92.4 - 100%）的耐药性增加（无统计学意义）。根据抗菌多重耐药性，将菌株分为三组：I. 对2 - 5种抗菌药物耐药；II. 对6 - 8种抗菌药物耐药；III. 对9 - 12种抗菌药物耐药。属于第一组的分离株数量显示出统计学意义上的下降（P < 0.05；R(2) = 0.896；r = -0.9608），而属于第二组和第三组的分离株在研究期间耐药性显示出统计学意义上的增加（分别为P < 0.05；R(2) = 0.753；r = 0.8890和P < 0.05；R(2) = 0.727；r = 0.8701）。本研究结果表明需要持续监测耐药性的发展情况
