Zhang Xiao-Mei, Shan Ning-Ning, Hu Yu, Wang Xin
Department of Hematology, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China.
Zhonghua Xue Ye Xue Za Zhi. 2013 Jul;34(7):614-7. doi: 10.3760/cma.j.issn.0253-2727.2013.07.012.
To explore the expression and clinical significance of T cell immunoglobulin mucin (TIM)-1, TIM-3 and T cell-specific transcription factors T-bet and GATA-3 in spleen mononuclear cells in patients with primary immune thrombocytopenia (ITP).
The spleen samples were obtained from 17 active ITP patients and 10 controls with spleen traumatic rupture. By using real-time quantitative polymerase chain reaction, the mRNA expressions of TIM-3, TIM1, T-bet and GATA-3 were studied in all subjects.
TIM-3 mRNA levels of active ITP patients were significantly decreased to (29 ± 16)% of that of control, TIM-1 mRNA levels of active ITP patients increased to (3.20 ± 2.18) folds of that of control, but the difference was not significant. The ratio of TIM-1/ TIM-3 was elevated in active ITP patients. T-bet mRNA levels were up-regulated in ITP patients by (2.82 ± 1.57) folds (P<0.05) and the expression of GATA3 was decreased by 14% folds (P<0.05) compared to controls. The ratio of T-bet/GATA3 were significantly elevated in ITP patients.
The imbalance between TIM-3 and TIM-1 expression might play an important role in pathogenesis of ITP.
探讨原发性免疫性血小板减少症（ITP）患者脾脏单个核细胞中T细胞免疫球蛋白黏蛋白（TIM）-1、TIM-3及T细胞特异性转录因子T-bet和GATA-3的表达及临床意义。
收集17例活动性ITP患者及10例脾脏外伤性破裂患者的脾脏标本，采用实时定量聚合酶链反应检测所有研究对象中TIM-3、TIM-1、T-bet和GATA-3的mRNA表达。
活动性ITP患者TIM-3 mRNA水平显著降低至对照组的（29±16）%，活动性ITP患者TIM-1 mRNA水平升高至对照组的（3.20±2.18）倍，但差异无统计学意义。活动性ITP患者TIM-1/TIM-3比值升高。与对照组相比，ITP患者T-bet mRNA水平上调（2.82±1.57）倍（P<0.05），GATA3表达下降14%（P<0.05）。ITP患者T-bet/GATA3比值显著升高。
TIM-3与TIM-1表达失衡可能在ITP发病机制中起重要作用。
