Lyons Scott K, Patrick P Stephen, Brindle Kevin M
Department of Molecular Imaging, CRUK Cambridge Research Institute, Li Ka Shing Centre, Cambridge CB2 0RE, United Kingdom.
Cold Spring Harb Protoc. 2013 Aug 1;2013(8):685-99. doi: 10.1101/pdb.top069864.
Imaging mouse models of cancer with reporter transgenes has become a relatively common experimental approach in the laboratory, which allows noninvasive and longitudinal investigation of diverse aspects of tumor biology in vivo. Our goal here is to outline briefly the principles of the relevant imaging modalities, emphasizing particularly their strengths and weaknesses and what the researcher can expect in a practical sense from each of these techniques. Furthermore, we discuss how relatively subtle modifications in the way reporter transgene expression is regulated in the cell underpin the ability of reporter transgenes as a whole to provide readouts on such varied aspects of tumor biology in vivo.
利用报告基因转基因对癌症小鼠模型进行成像已成为实验室中一种相对常见的实验方法，它能够在体内对肿瘤生物学的各个方面进行非侵入性的纵向研究。我们在此的目标是简要概述相关成像模式的原理，特别强调它们的优缺点以及研究人员从每种技术中实际可以期待的结果。此外，我们还将讨论在细胞中调节报告基因转基因表达的方式上相对细微的改变是如何支撑报告基因转基因作为一个整体在体内提供关于肿瘤生物学如此多方面的读数的能力的。
