Departamento de Ciencias Biomédicas, Instituto de Ciencias Biomédicas, Universidad CEU Cardenal Herrera, Avda. Seminario s/n, 46113 Moncada, Valencia, Spain.
Exp Eye Res. 2013 Oct;115:199-205. doi: 10.1016/j.exer.2013.07.011. Epub 2013 Jul 29.
We describe a procedure in which tears, obtained from Schirmer strips, are used to measure a marker of lipid peroxidation, malondialdehyde (MDA). We also compared the levels of proteins and MDA in tears from two groups of people: young adults (18-30 years old) and elderly adults (65-85 years old), because the data related to total protein concentration of human tears vary widely and because the majority of people over the age of 65 experience some symptoms of dry eyes and this condition has been recognized as an oxidative stress-induced disease. Our results show a significant difference in the protein concentration of the tears taken from the two age categories, younger adults (18-30 years old) and older adults (65-85 years old). Herein, we report for the first time an increase in MDA concentrations determined by HPLC in human tears based on age. It is possible that alterations in the tear lipid layer may lead to an increase in lipid peroxidation. Further studies are needed to understand the nature and function of tear film and stability in order to obtain new methods to analyze tears in patients with different diseases. In this sense, it would be interesting to compare MDA concentration in tears from control subjects and from people with meibomian gland dysfunction.
我们描述了一种使用 Schirmer 带获得的眼泪来测量脂质过氧化标志物丙二醛（MDA）的方法。我们还比较了两组人群（18-30 岁的年轻人和 65-85 岁的老年人）的眼泪中的蛋白质和 MDA 水平，因为与人类眼泪的总蛋白浓度相关的数据差异很大，而且大多数 65 岁以上的人都有一些干眼症的症状，这种情况已被认为是一种氧化应激诱导的疾病。我们的结果表明，从两个年龄段（年轻人 18-30 岁和老年人 65-85 岁）中采集的眼泪的蛋白质浓度存在显著差异。在此，我们首次报道了基于年龄的 HPLC 法测定人眼泪中 MDA 浓度的增加。眼泪中脂质层的改变可能导致脂质过氧化增加。需要进一步的研究来了解泪膜的性质和功能以及稳定性，以便获得分析不同疾病患者眼泪的新方法。从这个意义上说，比较对照组和睑板腺功能障碍患者眼泪中的 MDA 浓度将是很有趣的。
