Centre de Recherche des Cordeliers, INSERM, Université de Paris, Université Paris Sorbonne, From physiopathology of ocular diseases to clinical development, Paris, France.
Ophthalmology Department, Necker-Enfants Malades University Hospital, AP-HP, Paris, France.
Mol Vis. 2020 Oct 15;26:722-730. eCollection 2020.
Central serous chorioretinopathy (CSCR) has been associated with oxidative stress-related risk factors. The objective of this study was to optimize an analytical method for evaluating the oxidative stress biomarker malondialdehyde (MDA) in human tears and determine its level in the tears of patients with CSCR.
In this pilot study, tear samples were obtained from 34 healthy donors and 31 treatment-naïve CSCR male patients (eight with acute CSCR and 23 with chronic CSCR). Two analytical methods based on high-performance liquid chromatography followed by fluorescence detection were evaluated, with either 2-thiobarbituric derivative (TBA) or 2-aminoacridone (2-AA). Activity of CSCR was defined by the serous retinal detachment (SRD) height, which was measured by two independent observers on spectral-domain optical coherence tomography.
The 2-AA method showed higher sensitivity and precision compared to the TBA method. When the 2-AA method was applied to tears from healthy donors, the levels of MDA were statistically significantly higher in men compared to women (mean ± standard deviation, SD: 9,914 nM ± 6,126 versus 4,635 nM ± 1,173, p = 0.006). No difference was found in tear MDA levels between male patients with CSCR and age-matched control men (p = 0.17). However, MDA levels were statistically significantly higher in acute compared to chronic CSCR cases (mean ± SD: 12,295 nM ± 8,495 versus 6,790 ± 3,969 nM, p = 0.03). Additionally, there was a correlation between MDA levels and RPE leakage, quantified by the height of the serous retinal detachment (p = 0.02, r = 0.40).
Levels of MDA in tears, measured with an optimized analytical method, correlate with RPE leakage in CSCR.
中心性浆液性脉络膜视网膜病变（CSCR）与氧化应激相关的危险因素有关。本研究的目的是优化一种分析方法，以评估人眼的氧化应激生物标志物丙二醛（MDA），并确定其在 CSCR 患者泪液中的水平。
在这项初步研究中，从 34 名健康供体和 31 名未经治疗的 CSCR 男性患者（8 名急性 CSCR 和 23 名慢性 CSCR）中获得泪液样本。评估了两种基于高效液相色谱法（HPLC）后荧光检测的分析方法，分别使用 2-硫代巴比妥酸衍生物（TBA）或 2-氨基吖啶酮（2-AA）。CSCR 的活动通过两名独立观察者在光谱域光学相干断层扫描（SD-OCT）上测量的浆液性视网膜脱离（SRD）高度来定义。
与 TBA 法相比，2-AA 法显示出更高的灵敏度和精密度。当将 2-AA 法应用于健康供体的泪液时，男性的 MDA 水平明显高于女性（平均值±标准差，SD：9914 nM±6126 与 4635 nM±1173，p=0.006）。CSCR 男性患者与年龄匹配的男性对照之间的泪液 MDA 水平没有差异（p=0.17）。然而，急性 CSCR 病例的 MDA 水平明显高于慢性 CSCR 病例（平均值±SD：12295 nM±8495 与 6790 nM±3969 nM，p=0.03）。此外，MDA 水平与 RPE 渗漏之间存在相关性，由浆液性视网膜脱离的高度来定量（p=0.02，r=0.40）。
用优化的分析方法测量的泪液 MDA 水平与 CSCR 中的 RPE 渗漏相关。
