Functional Foods Forum, University of Turku, 20014 Turku, Finland.
Department of Microbiology, University of Stellenbosch, 7600 Stellenbosch, South Africa.
Int J Syst Evol Microbiol. 2013 Dec;63(Pt 12):4502-4507. doi: 10.1099/ijs.0.052985-0. Epub 2013 Aug 1.
Three lactic acid bacteria were isolated from faeces of a jackal (Canis mesomelas) and raccoons (Procyron lotor). The isolates formed a subcluster in the Lactobacillus salivarius phylogenetic group, closely related to Lactobacillus animalis, Lactobacillus apodemi and Lactobacillus murinus, by phylogenetic analysis based on 16S rRNA and recA gene sequences. Levels of DNA-DNA relatedness revealed that the isolates belonged to the same taxon and were genetically separated from their phylogenetic relatives. The three strains were non-motile, obligately homofermentative and produced l-lactic acid as the main end-product from d-glucose. The strains metabolized raffinose. The major cellular fatty acids in the three strains were C16 : 0, C18 : 1ω9c and C19 : 1 cyclo 9,10. Based on the data provided, it is concluded that the three strains represent a novel species of the genus Lactobacillus, for which the name Lactobacillus faecis sp. nov. is proposed. The type strain is AFL13-2(T) ( = JCM 17300(T) = DSM 23956(T)).
从豺（Canis mesomelas）和浣熊（Procyon lotor）的粪便中分离出了 3 株乳酸菌。通过基于 16S rRNA 和 recA 基因序列的系统发育分析，这些分离株形成了乳杆菌唾液亚种的一个亚群，与动物乳杆菌、副干酪乳杆菌和鼠乳杆菌密切相关。DNA-DNA 同源性水平表明，这些分离株属于同一分类群，与它们的系统发育亲缘关系在遗传上是分离的。这 3 株菌均为非运动性、严格同型发酵且从 d-葡萄糖产生 l-乳酸为主产物。这些菌株可代谢棉子糖。这 3 株菌的主要细胞脂肪酸为 C16:0、C18:1ω9c 和 C19:1 环 9,10。基于提供的数据，得出结论，这 3 株菌代表了乳杆菌属的一个新种，提议将其命名为粪肠球菌（Lactobacillus faecis sp. nov.）。其模式菌株为 AFL13-2(T)（=JCM 17300(T)=DSM 23956(T)）。
