Influence of Polysaccharides From on Short-Chain Fatty Acid Production and Quorum Sensing in .

Polysaccharide is one of the main active ingredients of , which has been proven to regulate the balance of gut microbiota. For the first time, this study focused on the regulation of polysaccharides from (PS) on , a specific probiotic in the intestinal tract. PS effectively promoted the biomass, biofilm and acetic acid production in 2-84, and enhanced quorum sensing (QS) signaling. The characteristics of gene sequence were analyzed using genomics approaches, and 2-84 was found to encode 18 genes that are closely related to QS and 10 genes related to short-chain fatty acids (SCFAs). Additionally, transcriptome and proteome analysis demonstrated that PS could promote the QS system of by enhancing the transcription of gene and expression of oppD protein. PS also regulated the production and metabolism of SCFAs of by upregulating the expression of and gene and adh2 protein, and downregulating the expression of gene. In conclusion, it was speculated that PS could affect intestinal SCFAs production by affecting the QS system and SCFAs production in . The present study implied that PS might have a role in promoting the growth of intestinal probiotics, where the QS system and SCFAs might be two of the important mechanisms for the probiotic activity of PS.