The People's Hospital of Zhengzhou University, Zhengzhou, 450003, China.
Mol Biol Rep. 2013 Sep;40(9):5469-75. doi: 10.1007/s11033-013-2645-9. Epub 2013 Aug 2.
SynCAM, also named by TSLC1, SgIGSF and IGSF4, was identified as a neural tissue-specific immunoglobulin-like cell-cell adhesion molecule. However, the role of SynCAM in tumorigenesis remains elusive. We aimed to clarify its epigenetic regulation and biological functions in glioblastoma. SynCAM was silenced in 72 % (5/7) glioblastoma cell lines. A significant downregulation was also detected in paired glioblastoma tumors compared with adjacent non-cancerous tissues. In contrast, SynCAM was readily expressed in various normal adult brain tissues. Ectopic expression of SynCAM in the silenced cancer cell line T98G significantly reduced colony formation and cell proliferation, induced cell cycle arrests and repressed cell invasive ability. Nude mice were subcutaneously injected into the flank with T98G cells and treated with normal saline, pcDNA3.1 (vector) or pcDNA3.1-SynCAM, respectively. Treatment with pcDNA3.1-SynCAM retarded growth in the xenografts, which contributed to a 58 % decrease in tumor volume compared to controls. In conclusion, our results suggest that SynCAM suppressions growth of glioblastoma and may serve as a novel functional tumor-suppressor gene.
SynCAM，也称为 TSLC1、SgIGSF 和 IGSF4，被鉴定为一种神经组织特异性免疫球蛋白样细胞-细胞黏附分子。然而，SynCAM 在肿瘤发生中的作用仍不清楚。我们旨在阐明其在胶质母细胞瘤中的表观遗传调控和生物学功能。SynCAM 在 72%（5/7）的胶质母细胞瘤细胞系中被沉默。与相邻的非癌组织相比，配对的胶质母细胞瘤肿瘤中也检测到明显下调。相比之下，SynCAM 在各种正常的成人脑组织中容易表达。SynCAM 在沉默的癌细胞系 T98G 中的异位表达显著降低了集落形成和细胞增殖，诱导了细胞周期停滞并抑制了细胞侵袭能力。裸鼠在侧腹皮下注射 T98G 细胞，并分别用生理盐水、pcDNA3.1（载体）或 pcDNA3.1-SynCAM 处理。pcDNA3.1-SynCAM 的处理延缓了异种移植物的生长，与对照组相比，肿瘤体积减少了 58%。总之，我们的结果表明 SynCAM 抑制胶质母细胞瘤的生长，可能作为一种新的功能性肿瘤抑制基因。
