Kong Fanqiang, Li Xiaoqing, Li Shuhong, Sheng Dan, Li Wenhu, Song Mingming
Department of Emergency, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong 264000, P.R. China.
Department of Obstetrics and Gynecology, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong 264000, P.R. China.
Oncol Lett. 2021 Feb;21(2):103. doi: 10.3892/ol.2020.12364. Epub 2020 Dec 10.
Glioblastoma (GBM) is a type of malignant tumor occurring in the brain that severely influences the life of affected individuals. GBM cells are highly infiltrative, which is one of the main obstacles in the treatment of the disease. Numerous microRNAs (miRNAs/miRs) are associated with the development of GBM. However, the effects of miR-15a-5p on GBM remain elusive. In the present study, reverse transcription-quantitative PCR and western blot analysis were applied for the detection of RNA and protein levels, respectively. Cell Counting Kit-8 and Transwell assays were performed to examine cell proliferation and invasion, respectively. TargetScan 7.1 and dual-luciferase reporter assay were utilized for the prediction and verification of the association between miRNAs and mRNAs. The present study revealed that miR-15a-5p expression was upregulated in the GBM T98G cell line. The results further demonstrated that, through the inhibition of cell adhesion molecule 1 expression and the promotion of Akt phosphorylation, miR-15a-5p was able to promote GBM cell proliferation and invasion. Overall, the present findings revealed a novel mechanism responsible for the development of GBM and provided an experimental basis for the diagnosis and treatment of GBM.
胶质母细胞瘤（GBM）是一种发生于脑部的恶性肿瘤，严重影响患者的生活。GBM细胞具有高度浸润性，这是该疾病治疗的主要障碍之一。众多微小RNA（miRNA/miR）与GBM的发生发展相关。然而，miR-15a-5p对GBM的影响仍不清楚。在本研究中，分别应用逆转录定量PCR和蛋白质印迹分析检测RNA和蛋白质水平。分别进行细胞计数试剂盒-8（CCK-8）和Transwell实验检测细胞增殖和侵袭能力。利用TargetScan 7.1和双荧光素酶报告基因实验预测和验证miRNA与mRNA之间的关联。本研究显示，GBM T98G细胞系中miR-15a-5p表达上调。结果进一步表明，miR-15a-5p通过抑制细胞黏附分子1的表达和促进Akt磷酸化，能够促进GBM细胞增殖和侵袭。总体而言，本研究结果揭示了一种导致GBM发生发展的新机制，并为GBM的诊断和治疗提供了实验依据。
