Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou, 510650, People's Republic of China.
Antonie Van Leeuwenhoek. 2013 Nov;104(5):715-24. doi: 10.1007/s10482-013-9979-0. Epub 2013 Aug 2.
An Fe(III) oxide-reducing bacterium designated as SgZ-3(T), which could couple glucose oxidation to Fe(III) oxide reduction for energy conservation, was isolated from an electrochemical biofilm. The isolate was Gram-negative, catalase-positive and oxidase-negative, and facultative anaerobic. NaCl was not required for growth, but NaCl concentrations up to 5 % (w/v) were tolerated. Growth occurred in TSB (tryptic soy broth) at 20-40 °C (optimum 30 °C) and at pH 6.0-7.5 (optimum 7.0). Phototrophic growth could not be demonstrated. No vesicular photosynthetic membrane was observed. Bacteriochlorophyll α and carotenoids were absent. Biotin and thiamine were required as growth factors for the isolate. Phylogenetic analysis of the 16S rRNA gene sequence placed strain SgZ-3(T) within the family Rhodobacteraceae and affiliated with an phototrophic genera Rhodobacter. The G+C content of the genomic DNA was 68.6 mol%. Strain SgZ-3(T) contained Q-10 as the predominant quinone. The major cellular fatty acids were C18:1 ω6c and/or C18:1 ω7c (66.9 %) and C16:0 (9.5 %). Based on its phenotypic and phylogenetic properties, chemotaxonomic analysis and the results of physiological and biochemical tests, strain SgZ-3(T) (=KACC 16603(T) = CCTCC AB2012026(T)) was designated as the type strain of a new genus and a novel species of the family Rhodobacteraceae, for which the name Sinorhodobacter ferrireducens sp. nov. was proposed.
一株可将葡萄糖氧化与氧化铁还原偶联以进行能量保存的氧化铁还原细菌 SgZ-3(T)，从电化学生物膜中分离得到。该分离株为革兰氏阴性、过氧化氢酶阳性、氧化酶阴性兼性厌氧菌。生长不需要 NaCl，但可耐受高达 5%（w/v）的 NaCl。在 TSB（胰蛋白酶大豆肉汤）中于 20-40°C（最佳 30°C）和 pH 6.0-7.5（最佳 7.0）下生长。不能证明光养生长。未观察到泡状光合膜。没有菌叶绿素 α 和类胡萝卜素。生物素和硫胺素是该分离株的生长因子。16S rRNA 基因序列的系统发育分析将菌株 SgZ-3(T)置于红杆菌科（Rhodobacteraceae）内，并与光养属 Rhodobacter 相关联。基因组 DNA 的 G+C 含量为 68.6 mol%。菌株 SgZ-3(T)含有 Q-10 作为主要醌。主要细胞脂肪酸为 C18:1 ω6c 和/或 C18:1 ω7c（66.9%）和 C16:0（9.5%）。基于其表型和系统发育特性、化学分类分析以及生理生化测试的结果，菌株 SgZ-3(T)（=KACC 16603(T)=CCTCC AB2012026(T)）被指定为红杆菌科一个新属和一个新种的模式株，该新种命名为 Sinorhodobacter ferrireducens sp. nov.。
