Shang D Q
Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing.
Zhonghua Liu Xing Bing Xue Za Zhi. 1990 Jun;11(3):160-6.
This paper reports a comprehensive study on taxonomy of Brucella. In which 17 atypical and R phase strains were identified by using the routine methods, 6 groups of phages and oxidative metabolic tests. 4 McAb of Brucella were developed and applied for identification. Meanwhile OMP profiles of S phase of B. abortus, B. melitensis and B. suis were compared during identification of them. The results show that a comprehensive identification with routine methods, phage-typing and oxidative metabolic tests are able to determine these strains if they are species or biotypes. Several strains of Brucella McAb and OMP profiles of Brucella were firstly applied for identification of atypical and R strains, and provided some possibility in the application of identification of Brucella by using these two methods.
本文报道了一项关于布鲁氏菌分类学的综合研究。通过常规方法、6组噬菌体及氧化代谢试验鉴定出17株非典型菌和R相菌。研制了4种布鲁氏菌单克隆抗体并应用于鉴定。同时，在流产布鲁氏菌、羊布鲁氏菌和猪布鲁氏菌S相的鉴定过程中比较了它们的外膜蛋白图谱。结果表明，采用常规方法、噬菌体分型和氧化代谢试验进行综合鉴定能够确定这些菌株是种还是生物型。首次将几株布鲁氏菌单克隆抗体和布鲁氏菌外膜蛋白图谱应用于非典型菌和R相菌的鉴定，为利用这两种方法进行布鲁氏菌鉴定的应用提供了一些可能性。
