Department of Physiology and Biophysics, Boston University School of Medicine, Boston, Massachusetts 02118.
Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California 94301.
J Biol Chem. 2013 Sep 20;288(38):27287-27298. doi: 10.1074/jbc.M113.498899. Epub 2013 Aug 1.
Infection with many positive-strand RNA viruses dramatically remodels cellular membranes, resulting in the accumulation of double-membraned vesicles that resemble cellular autophagosomes. In this study, a single protein encoded by poliovirus, 3AB, is shown to be sufficient to induce the formation of double-membraned liposomes via the invagination of single-membraned liposomes. Poliovirus 3AB is a 109-amino acid protein with a natively unstructured N-terminal domain. HeLa cells transduced with 3AB protein displayed intracellular membrane disruption; specifically, the formation of cytoplasmic invaginations. The ability of a single viral protein to produce structures of similar topology to cellular autophagosomes should facilitate the understanding of both cellular and viral mechanisms for membrane remodeling.
许多正链 RNA 病毒的感染会显著重塑细胞膜，导致双层膜囊泡的积累，这些囊泡类似于细胞自噬体。在这项研究中，脊髓灰质炎病毒编码的单一蛋白 3AB 被证明足以通过单膜囊泡的内陷诱导双层膜脂质体的形成。脊髓灰质炎病毒 3AB 是一种由 109 个氨基酸组成的蛋白质，其 N 端结构域天然无结构。用 3AB 蛋白转导的 HeLa 细胞显示出细胞内膜的破坏；具体来说，细胞质内陷的形成。单个病毒蛋白产生与细胞自噬体相似拓扑结构的能力应该有助于理解细胞和病毒的膜重塑机制。
