Plant Soil and Microbial Sciences Department, Michigan State University East Lansing, MI, USA.
Front Plant Sci. 2013 Jul 30;4:286. doi: 10.3389/fpls.2013.00286. eCollection 2013.
Zinc is an essential mineral for humans and plants and is involved in many physiological and biochemical processes. In humans, Zn deficiency has been associated with retarded growth and reduction of immune response. In plants, Zn is an essential component of more than 300 enzymes including RNA polymerase, alkaline phosphatase, alcohol dehydrogenase, Cu/Zn superoxidase dismutase, and carbonic anhydrase. The accumulation of Zn in plants involves many genes and characterization of the role of these genes will be useful in biofortification. Here we report the identification and phlyogenetic and sequence characterization of the 23 members of the ZIP (ZRT, IRT like protein) family of metal transporters and three transcription factors of the bZIP family in Phaseolus vulgaris L. Expression patterns of seven of these genes were characterized in two bean genotypes (G19833 and DOR364) under two Zn treatments. Tissue analyzed included roots and leaves at vegetative and flowering stages, and pods at 20 days after flowering. Four of the genes, PvZIP12, PvZIP13, PvZIP16, and Pv bZIP1, showed differential expression based on tissue, Zn treatment, and/or genotype. PvZIP12 and PvZIP13 were both more highly expressed in G19833 than DOR364. PvZIP12 was most highly expressed in vegetative leaves under the Zn (-) treatment. PvZIP16 was highly expressed in leaf tissue, especially leaf tissue at flowering stage grown in the Zn (-) treatment. Pv bZIP1 was most highly expressed in leaf and pod tissue. The 23 PvZIP genes and three bZIP genes were mapped on the DOR364 × G19833 linkage map. PvZIP12, PvZIP13, and PvZIP18, Pv bZIP2, and Pv bZIP3 were located near QTLs for Zn accumulation in the seed. Based on the expression and mapping results, PvZIP12 is a good candidate gene for increasing seed Zn concentration and increase understanding of the role of ZIP genes in metal uptake, distribution, and accumulation of zinc in P. vulgaris.
锌是人体和植物必需的矿物质，参与许多生理和生化过程。在人体中，锌缺乏与生长迟缓以及免疫反应降低有关。在植物中，锌是超过 300 种酶的必需组成部分，包括 RNA 聚合酶、碱性磷酸酶、乙醇脱氢酶、Cu/Zn 超氧化物歧化酶和碳酸酐酶。植物中锌的积累涉及许多基因，这些基因的特征描述将有助于生物强化。在这里，我们报告了在菜豆中鉴定和系统发育及序列特征 23 个金属转运体 ZIP（ZRT、IRT 样蛋白）家族成员和 3 个 bZIP 家族转录因子。在两种锌处理下，两种菜豆基因型（G19833 和 DOR364）中对其中 7 个基因的表达模式进行了特征描述。分析的组织包括营养生长和开花阶段的根和叶，以及开花后 20 天的豆荚。其中 4 个基因，PvZIP12、PvZIP13、PvZIP16 和 Pv bZIP1，根据组织、锌处理和/或基因型表现出差异表达。与 DOR364 相比，G19833 中 PvZIP12 和 PvZIP13 的表达水平更高。在 Zn（-）处理下，PvZIP12 在营养叶中表达水平最高。PvZIP16 在叶组织中高度表达，尤其是在 Zn（-）处理下开花期的叶组织中。Pv bZIP1 在叶组织和豆荚组织中表达水平最高。23 个 PvZIP 基因和 3 个 bZIP 基因被映射到 DOR364×G19833 连锁图谱上。PvZIP12、PvZIP13 和 PvZIP18、Pv bZIP2 和 Pv bZIP3 位于种子中锌积累的 QTL 附近。基于表达和图谱结果，PvZIP12 是提高种子锌浓度的候选基因，增加了对 ZIP 基因在菜豆金属吸收、分配和锌积累中的作用的理解。
