Saavedra Núñez Germán, González-Villanueva Enrique, Ramos Patricio
Instituto de Ciencias Biológicas, Universidad de Talca, Talca 3460787, Chile.
Vicerrectoría de Investigación y Postgrado, Universidad Católica del Maule, Talca 3480112, Chile.
Plants (Basel). 2023 Sep 20;12(18):3322. doi: 10.3390/plants12183322.
Several grapevine ( L.) cultivars show a tendency to develop parthenocarpic seedless grapes, affecting fruit yield and quality. This reproductive disorder originates in defective ovule fertilization due to a failure in pollen tube growth. Zinc (Zn) is a crucial trace element, playing a vital role in various physiological and metabolic processes. It is particularly essential for the healthy growth of flowers and fruits. Insufficient zinc has been suggested as a potential reason for issues in this development process. This microelement is taken up through a mechanism that involves transporters, including the ZRT-IRT-like protein (ZIP) gene family, associated with the influx of Zn into the cell. In grapevines, 20 genes for ZIP-type transporters have been described. In this study, we analyzed the expression pattern of during flower development and employ transgenic methods to assess its transcriptional regulation. Furthermore, through computational examination of the promoter region, we identified two CArG boxes, recognized as responsive elements to MADS transcription factors. These factors play a key role in shaping various components of a flower, such as pollen. Our investigation of the promoter confirms the functionality of these CArG boxes. Overall, our results suggest that the increased expression of during flowering is likely under the influence of MADS transcription factors.
几个葡萄（Vitis vinifera L.）品种表现出形成单性结实无核葡萄的倾向，这会影响果实产量和品质。这种生殖障碍源于花粉管生长失败导致的胚珠受精缺陷。锌（Zn）是一种关键的微量元素，在各种生理和代谢过程中发挥着至关重要的作用。它对花和果实的健康生长尤为重要。锌不足被认为是这一发育过程中出现问题的一个潜在原因。这种微量元素是通过一种涉及转运蛋白的机制吸收的，其中包括与锌流入细胞相关的ZRT-IRT类蛋白（ZIP）基因家族。在葡萄中，已经描述了20个ZIP型转运蛋白基因。在本研究中，我们分析了[具体基因名称未给出]在花发育过程中的表达模式，并采用转基因方法评估其转录调控。此外，通过对启动子区域的计算分析，我们鉴定出两个CArG盒，它们被认为是MADS转录因子的响应元件。这些因子在塑造花的各种组成部分（如花粉）中起关键作用。我们对[具体基因名称未给出]启动子的研究证实了这些CArG盒的功能。总体而言，我们的结果表明，开花期间[具体基因名称未给出]表达的增加可能受到MADS转录因子的影响。
