College of Food Science, Fujian Agriculture and Forestry Univ. Fuzhou, Fujian 350002, China; Natl. Engineering Research Center of Juncao, Fuzhou, Fujian 350002, China.
J Food Sci. 2013 Sep;78(9):H1470-5. doi: 10.1111/1750-3841.12226. Epub 2013 Aug 1.
A water-soluble crude polysaccharide was extracted by hot water from Auricularia auricular mycelium grown under solid-state fermentation (SSF). The crude polysaccharide was purified by DEAE Sephadex A-50 and Sephadex G-200 chromatography. Fourier transform infrared spectroscopy and nuclear magnetic resonance ((1) H NMR) spectroscopy were used to investigate the structure of the purified A. auricular polysaccharide (AAP-I) and revealed that it is α-glycosidically linked. After 14 and 28 days of AAP-I orally administered, the AAP-I significantly decreased the levels of total cholesterol, triglyceride, and low-density lipoprotein cholesterol in mice in which hyperlipidemia had been induced by a high fat diet (P < 0.05). The results revealed that AAP-I from SSF of A. auricular mycelium possesses potent hypolipidemic properties. The polysaccharide may be useful as a functional food additive and a hypolipidemic agent.
一种水溶性粗多糖，采用热水法从固态发酵（SSF）培养的银耳菌丝体中提取。粗多糖通过 DEAE Sephadex A-50 和 Sephadex G-200 层析进行纯化。傅里叶变换红外光谱和核磁共振 ((1) H NMR) 光谱用于研究纯化的银耳多糖（AAP-I）的结构，表明它是α-糖苷键连接的。经口给予 AAP-I 14 和 28 天后，AAP-I 显著降低了高脂饮食诱导的高血脂小鼠的总胆固醇、甘油三酯和低密度脂蛋白胆固醇水平（P<0.05）。结果表明，银耳菌丝体 SSF 中的 AAP-I 具有较强的降血脂作用。该多糖可用作功能性食品添加剂和降血脂剂。
