a CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering , University of Science and Technology of China , Hefei , 230026 , P.R. China .
J Biomater Sci Polym Ed. 2013;24(18):2058-70. doi: 10.1080/09205063.2013.823072. Epub 2013 Aug 2.
This study describes the formation of highly efficient antiprotein adsorption random copolymer coating of poly(N,N-dimethylacrylamide-co-sulfobetaine methacrylate) (poly(DMA-co-SBMA)) on the fused-silica capillary inner wall. Firstly, the poly(DMA-co-SBMA)s with different feed ratio (SBMA/DMA) were synthesized via the reversible addition fragmentation chain transfer polymerization. And then, X-ray photoelectron spectroscopy (XPS) and water contact angle (CA) were used to investigate the composition and hydrophilicity of poly(DMA-co-SBMA) coating formed on the glass slide surfaces. CA measurements revealed that the poly(DMA-co-SBMA) coating became more hydrophilic with the increment of feed ratio (SBMA/DMA), and at the same time, the XPS results showed that the coating ability was also increased with the increment of feed ratio. Followed, the copolymer was applied to coat the fused-silica capillary inner wall, and the coated capillary was used to separate the mixture of proteins (lysozyme, cytochrome c, ribonuclease A, and α-chymotrypsinogen A) in a pH range from 3.0 to 5.0. Under the optimum conditions, an excellent separation of basic proteins with peak efficiencies ranging from 551,000 to 1509,000 N/m had been accomplished within 10 min. Furthermore, the effect of coating composition on protein separation was also investigated through the comparison of separation efficiency achieved by using bare, PSBMA- and poly(DMA-co-SBMA)-coated capillary, respectively.
本研究描述了在熔融石英毛细管内壁上形成高效抗蛋白吸附的聚（N,N-二甲基丙烯酰胺-co-磺基甜菜碱甲基丙烯酸酯）（poly(DMA-co-SBMA)）无规共聚物涂层的过程。首先，通过可逆加成-断裂链转移聚合合成了不同进料比（SBMA/DMA）的 poly(DMA-co-SBMA)。然后，通过 X 射线光电子能谱（XPS）和水接触角（CA）研究了在玻璃片表面形成的 poly(DMA-co-SBMA)涂层的组成和亲水性。CA 测量表明，随着进料比（SBMA/DMA）的增加，poly(DMA-co-SBMA)涂层的亲水性增加，同时 XPS 结果表明，涂层能力也随进料比的增加而增加。随后，将共聚物应用于涂覆熔融石英毛细管内壁，并使用涂覆的毛细管在 pH 值为 3.0 至 5.0 的范围内分离蛋白质混合物（溶菌酶、细胞色素 c、核糖核酸酶 A 和α-糜蛋白酶原 A）。在最佳条件下，在 10 分钟内实现了碱性蛋白质的出色分离，峰效率范围从 551,000 到 1509,000 N/m。此外，还通过比较使用裸、PSBMA-和 poly(DMA-co-SBMA)-涂覆毛细管分别获得的分离效率，研究了涂层组成对蛋白质分离的影响。
