Chen Lijuan, Tan Lin, Liu Songtao, Bai Longchao, Wang Yanmei
a CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering , University of Science and Technology of China , Hefei 230026 , P.R. China.
J Biomater Sci Polym Ed. 2014;25(8):766-85. doi: 10.1080/09205063.2014.905030. Epub 2014 Apr 8.
In this paper, a novel copolymer consisting of sulfobetaine methacrylate (SBMA) and 2-aminoethyl methacrylate (AEMA) named as poly(SBMA-co-AEMA) was synthesized by conventional free-radical polymerization, the poly(SBMA-co-AEMA) zwitterionic copolymer was immobilized onto glass slides surface through polydopamine (PDA)-anchored coating and formed poly(SBMA-co-AEMA)-g-PDA coating. The defined copolymer was characterized by nuclear magnetic resonance hydrogen spectroscopy ((1)H NMR) and gel permeation chromatography. The surface morphology, thickness, and chemical component of poly(SBMA-co-AEMA)-g-PDA coating were studied by atom force microscope, ellipsometry, and X-ray photoelectron spectroscopy, respectively. The hydrophilicity and stability of these coatings were investigated by static water contact angles. And finally, the poly(SBMA-co-AEMA)-g-PDA coating was successfully applied into capillary inner surface for suppression electro-osmotic flow and protein separation by capillary electrophoresis.
在本文中，通过常规自由基聚合合成了一种由甲基丙烯酸磺酸甜菜碱（SBMA）和甲基丙烯酸2-氨基乙酯（AEMA）组成的新型共聚物，命名为聚（SBMA-co-AEMA），聚（SBMA-co-AEMA）两性离子共聚物通过聚多巴胺（PDA）锚定涂层固定在载玻片表面，形成聚（SBMA-co-AEMA）-g-PDA涂层。通过核磁共振氢谱（¹H NMR）和凝胶渗透色谱对所定义的共聚物进行了表征。分别用原子力显微镜、椭偏仪和X射线光电子能谱研究了聚（SBMA-co-AEMA）-g-PDA涂层的表面形貌、厚度和化学成分。通过静态水接触角研究了这些涂层的亲水性和稳定性。最后，聚（SBMA-co-AEMA）-g-PDA涂层成功应用于毛细管内表面，用于抑制毛细管电泳中的电渗流和蛋白质分离。
