Department of Ear, Nose and Throat, University Hospital Schleswig-Holstein, Luebeck, Germany.
Nutr Cancer. 2013;65(6):843-9. doi: 10.1080/01635581.2013.804579.
In head and neck squamous cell carcinoma (HNSCC) aerobic glycolysis is the key feature for energy supply of the tumor. Quantitative microdialysis (μD) offers an online method to measure parameters of the carbohydrate metabolism in vivo. The aim was to standardize a quantitative μD-study in patients with HNSCC and to prove if a ketogenic diet would differently influence the carbohydrate metabolism of the tumor tissue. Commercially available 100 kDa-CMA71-μD- catheters were implanted in tumor-free and in tumor tissue in patients with HNSCC for simultaneous measurements up to 5 days. The metabolic pattern and circadian rhythm of urea, glucose, lactate, and pyruvate was monitored during 24 h of western diet and subsequent up to 4 days of ketogenic diet. After 3 days of ketogenic diet the mean lactate concentration declines to a greater extent in the tumor tissue than in the tumor-free mucosa, whereas the mean glucose and pyruvate concentrations rise. The in vivo glucose metabolism of the tumor tissue is clearly influenced by nutrition. The decline of mean lactate concentration in the tumor tissue after ketogenic diet supports the hypothesis that HNSCC tumor cells might use lactate as fuel for oxidative glucose metabolism.
在头颈部鳞状细胞癌（HNSCC）中，有氧糖酵解是肿瘤能量供应的关键特征。定量微透析（μD）提供了一种在线方法来测量体内碳水化合物代谢的参数。目的是标准化 HNSCC 患者的定量 μD 研究，并证明生酮饮食是否会对肿瘤组织的碳水化合物代谢产生不同的影响。将商业上可用的 100 kDa-CMA71-μD-导管植入 HNSCC 患者的无肿瘤组织和肿瘤组织中，以进行长达 5 天的同时测量。在西方饮食期间和随后的 4 天生酮饮食期间监测尿素、葡萄糖、乳酸和丙酮酸的代谢模式和昼夜节律。生酮饮食 3 天后，肿瘤组织中的乳酸浓度平均下降幅度大于无肿瘤黏膜，而葡萄糖和丙酮酸浓度平均上升。肿瘤组织中肿瘤组织的葡萄糖代谢明显受到营养的影响。生酮饮食后肿瘤组织中乳酸浓度的下降支持了 HNSCC 肿瘤细胞可能将乳酸用作氧化葡萄糖代谢燃料的假设。
