1 Department of Human Nutrition & Hygiene, Poznan University of Life Sciences , Poznan, Poland .
J Med Food. 2013 Sep;16(9):847-56. doi: 10.1089/jmf.2012.0168. Epub 2013 Aug 3.
In this study, a most consumer-acceptable rye bread (RB) containing saffron (S) powder (RB+S) was designed to verify its anti-diabetic properties, and to compare these effects with those of RB and S separately, matched to a similar dose of bioactive components, used in the high-fat (HF) diet in streptozotocin (STZ)-induced Wistar rats. After baking, beneficial antioxidant and sensory properties for RB enriched with 0.12% S were achieved. Twenty-four severely diabetic rats (fasting blood glucose (FBG) ≥350 mg/dL) were randomized to incorporate either 0.08% of pure S, or RB enriched with 0.12% S (the diet provided 0.08% of S), or RB alone into their diet for 5 weeks. As controls, nontreated, HF-feeding STZ-induced rats (positive control-HF/STZ) and rats receiving normal laboratory diet (negative control-C) were used. A significant FBG-lowering effect was observed (47%, 53%, and 54% reduction vs. HF/STZ; P<.05) after S, RB, and RB+S treatment. Improvements in the rats' glycemia were achieved by β-cell regeneration and increases in insulin secretion. Only in the S and RB+S group of rats, a significant (P<.05) increase in relative pancreas (vs. HF/STZ) was noted. A significant (P<.05) reduction in the concentration of thiobarbituric acid-reactive substances (TBARS) was achieved, whereas the ferric-reducing ability of plasma (FRAP) was not changed after S, RB and RB+S treatment (vs. HF/STZ). Triglyceride (TG) concentrations after S, RB, and RB+S treatment were significantly decreased (P<.05) versus HF/STZ. Both S and RB can be used in diabetic therapy, but no additional metabolic effect was achieved after consumption of RB+S.
在这项研究中，设计了一种最受消费者接受的含藏红花（S）粉的黑麦面包（RB+S），以验证其抗糖尿病特性，并将这些效果与 RB 和 S 分别进行比较，两者均匹配到高脂肪（HF）饮食中链脲佐菌素（STZ）诱导的 Wistar 大鼠中使用的类似剂量的生物活性成分。经过烘焙，富含 0.12%S 的 RB 实现了有益的抗氧化和感官特性。24 只严重糖尿病大鼠（空腹血糖（FBG）≥350mg/dL）被随机分为三组，分别摄入 0.08%的纯 S、富含 0.12%S 的 RB（饮食提供 0.08%的 S）或单独的 RB 5 周。作为对照，未治疗的 HF 喂养 STZ 诱导的大鼠（阳性对照-HF/STZ）和接受正常实验室饮食的大鼠（阴性对照-C）被使用。S、RB 和 RB+S 处理后，观察到 FBG 显著降低（分别降低 47%、53%和 54%，与 HF/STZ 相比，P<.05）。通过β细胞再生和增加胰岛素分泌，改善了大鼠的血糖水平。只有在 S 和 RB+S 组的大鼠中，观察到相对胰腺（与 HF/STZ 相比）显著增加（P<.05）。S、RB 和 RB+S 处理后，硫代巴比妥酸反应物质（TBARS）的浓度显著降低（P<.05），而血浆铁还原能力（FRAP）没有变化（与 HF/STZ 相比）。S、RB 和 RB+S 处理后，甘油三酯（TG）浓度显著降低（P<.05）与 HF/STZ 相比。S 和 RB 均可用于糖尿病治疗，但消费 RB+S 后并未获得额外的代谢效果。
