胃癌患者幽门螺杆菌根除前后黏蛋白 MUC5AC 表达的变化。

The changes in MUC5AC expression in gastric cancer before and after Helicobacter pylori eradication.

机构信息

Department of Gastroenterology, First People's Hospital of Yuhang District, Hangzhou 31100, Zhejiang Province, China.

Department of General Surgery, Central Hospital of Yiwu city, Jinhua 32100, Zhejiang Province, China.

出版信息

Clin Res Hepatol Gastroenterol. 2014 Apr;38(2):235-40. doi: 10.1016/j.clinre.2013.06.008. Epub 2013 Jul 30.

DOI:10.1016/j.clinre.2013.06.008

PMID:23910060

Abstract

PURPOSE

To investigate MUC5AC expression in gastric cancer before and after Hp eradication.

METHODS

The MUC5AC protein and mRNA were detected in gastric cancer tissue by western blot and real time PCR protocols before and after Hp eradication (Hp positive group). Gastric cancer tissue without Hp infection served as the control group (Hp negative group).

RESULTS

The MUC5AC protein and mRNA expression was more significantly increased in gastric cancer after Hp eradication as compared to that before Hp eradication, but it was significantly lower than of the control group. The relative amount of MUC5AC in the well-differentiated cancer was higher than that of the moderately or poorly-differentiated cancer, in either Hp positive or control groups. The relative amount of MUC5AC in cancer tissues with more than five metastatic lymph nodes was significantly lower than that of the cancer tissues with five or less metastatic lymph nodes, and was significantly lower in the Hp positive group as compared to that of the control group.

CONCLUSIONS

The reduction of the MUC5AC might be related to gastric carcinogenesis caused by Hp and the progression of gastric cancer.

摘要

目的

研究幽门螺杆菌（Hp）根除前后胃癌中 MUC5AC 的表达。

方法

采用 Western blot 和实时 PCR 方案检测 Hp 阳性组（Hp 阳性组）胃癌组织中 MUC5AC 蛋白和 mRNA 在 Hp 根除前后的表达。无 Hp 感染的胃癌组织作为对照组（Hp 阴性组）。

结果

与 Hp 根除前相比，Hp 根除后胃癌中 MUC5AC 蛋白和 mRNA 的表达明显增加，但明显低于对照组。在 Hp 阳性或对照组中，高分化癌的 MUC5AC 相对量高于中低分化癌。在转移淋巴结超过 5 个的癌组织中，MUC5AC 的相对量明显低于转移淋巴结为 5 个或更少的癌组织，且 Hp 阳性组明显低于对照组。