Department of Oral Biology and Experimental Dental Research, Faculty of Dentistry, University of Szeged, H-6720 Szeged, Hungary.
Mater Sci Eng C Mater Biol Appl. 2013 Oct;33(7):4251-9. doi: 10.1016/j.msec.2013.06.020. Epub 2013 Jun 26.
Demand is increasing for shortening the long (3-6 months) osseointegration period to rehabilitate patients' damaged chewing apparatus in as short a time as possible. For dental implants, as for biomaterials in general, the bio- and osseointegration processes can be controlled at molecular and cellular levels by modification of the implant surface. One of the most promising of such surface modifications is laser ablation, as demonstrated by our previous results [46]. Commercially pure (CP4) sand-blasted, acid-etched titanium disks (Denti® System Ltd., Hungary) were irradiated with a KrF excimer laser (248 nm, fluence 0.4 J/cm(2), FWHM 18 ns, 2000 pulses), or with a Nd:YAG laser (532 nm, 1.3 J/cm(2), 10 ns, 200 pulses) then examined by SEM, AFM, and XPS. In vitro attachment (24 h) and proliferation (72 h) of MG-63 osteoblast cells were investigated via dimethylthiazol-diphenyl tetrazolium bromide (MTT), alamarBlue (AB) assays alkaline phosphatase quantification (ALP) and SEM. SEM and AFM revealed significant changes in morphology and roughness. XPS confirmed the presence of TiO2 on each sample; after Nd:YAG treatment a reduced state of Ti (Ti(3+)) was also observed. MTT, AB and ALP measurements detected an increase in the number of cells between the 24- and 72 hour observations; however, laser treatment did not affect cell attachment and proliferation significantly.
需求正在增加，需要缩短长（3-6 个月）的骨整合期，以便尽快恢复患者受损的咀嚼器官。对于牙科植入物，就像一般的生物材料一样，可以通过修改植入物表面来控制生物和骨整合过程在分子和细胞水平上。表面改性中最有前途的方法之一是激光烧蚀，正如我们之前的结果[46]所示。商用纯（CP4）喷砂酸蚀钛盘（Denti® System Ltd.，匈牙利）用 KrF 准分子激光（248nm，剂量 0.4J/cm(2)，半峰全宽 18ns，2000 个脉冲）或 Nd:YAG 激光（532nm，1.3J/cm(2)，10ns，200 个脉冲）进行辐照，然后用 SEM、AFM 和 XPS 进行检查。通过二甲基噻唑二苯基四氮唑溴盐（MTT）、阿尔玛蓝（AB）测定碱性磷酸酶定量（ALP）和 SEM 研究了 MG-63 成骨细胞的附着（24h）和增殖（72h）。SEM 和 AFM 显示形貌和粗糙度发生了显著变化。XPS 证实了每个样品上都存在 TiO2；在 Nd:YAG 处理后，还观察到 Ti（Ti(3+)）的还原态。MTT、AB 和 ALP 测量检测到细胞数量在 24 小时至 72 小时观察期间增加；然而，激光处理并没有显著影响细胞的附着和增殖。
