Galli Carlo, Guizzardi Stefano, Passeri Giovanni, Martini Desiree', Tinti Anna, Mauro Giovanni, Macaluso Guido Maria
Periodontology Unit, University of Parma, Parma, Italy.
J Periodontol. 2005 Mar;76(3):364-72. doi: 10.1902/jop.2005.76.3.364.
Surface characteristics play a major role in determining tissue response to implants and therefore their clinical outcome. The aim of the present study was to compare two commercially available titanium surfaces: plasma sprayed (TPS) and sand-blasted, acid-etched surface (SLA).
The surfaces were characterized by roughness testing, scanning electronic microscopy (SEM), Raman spectroscopy, and protein adsorption to determine their microtopographic and chemical properties. The effect of the surfaces on human mandibular osteoblasts was then studied in terms of cell morphology, adhesion, proliferation, and differentiation. Human osteoblasts from the mandible were cultured on these two surfaces and evaluated at 3, 6, 24, and 48 hours to determine cell attachment and morphology. Growth and differentiation kinetics were subsequently investigated by evaluating cell growth, alkaline phosphatase activity, osteocalcin and osteoprotegerin production at 7, 14, and 21 days.
Although roughness was quite similar, the two surfaces presented strong differences in their topography, and cell morphology varied as a consequence. Osteoblasts on SLA appeared more elongated and spindle shaped than those on TPS, and their adhesion at 3 and 6 hours was weaker, but reached that of cells on TPS at hour 24. Cell proliferation was greater on SLA surfaces but differentiation parameters; i.e., alkaline phosphatase and osteocalcin, provided better results on TPS surfaces. Osteoprotegerin production was enhanced on TPS surfaces at days 14 and 21.
Although cells grown on both surfaces exhibited good adhesion capabilities, a well-differentiated osteoblastic phenotype, and maintained a clear proliferation potential, our study suggests that plasma-sprayed treatment offers a better performance than SLA by creating, at least in the early phases, better conditions for tissue healing.
表面特性在决定组织对植入物的反应及其临床结果方面起着主要作用。本研究的目的是比较两种市售钛表面：等离子喷涂（TPS）表面和喷砂酸蚀表面（SLA）。
通过粗糙度测试、扫描电子显微镜（SEM）、拉曼光谱和蛋白质吸附对表面进行表征，以确定其微观形貌和化学性质。然后从细胞形态、黏附、增殖和分化方面研究这些表面对人下颌骨成骨细胞的影响。将来自下颌骨的人成骨细胞培养在这两种表面上，并在3、6、24和48小时进行评估，以确定细胞附着和形态。随后通过在第7、14和21天评估细胞生长、碱性磷酸酶活性、骨钙素和骨保护素的产生来研究生长和分化动力学。
尽管粗糙度相当相似，但两种表面在形貌上存在很大差异，细胞形态也因此不同。与TPS表面上的成骨细胞相比，SLA表面上的成骨细胞显得更细长且呈纺锤形，其在3小时和6小时时的黏附较弱，但在24小时时达到了TPS表面上细胞的黏附水平。SLA表面上的细胞增殖更强，但分化参数，即碱性磷酸酶和骨钙素，在TPS表面上的结果更好。在第14天和21天，TPS表面上的骨保护素产生增加。
尽管在两种表面上生长的细胞均表现出良好的黏附能力、分化良好的成骨细胞表型并保持明显的增殖潜力，但我们的研究表明，至少在早期阶段，等离子喷涂处理通过为组织愈合创造更好的条件，比SLA表现更好。
