LISM EA 4695, Université de Reims Champagne-Ardenne, 21 rue Clément ADER, 51685 Reims, BP 138 Cedex 02, France.
Mater Sci Eng C Mater Biol Appl. 2013 Oct;33(7):4260-5. doi: 10.1016/j.msec.2013.06.019. Epub 2013 Jun 26.
Strontium-substituted calcium phosphate coatings are synthesized by pulsed electrodeposition on titanium alloy (Ti6Al4V) substrates. Experimental conditions of the process are optimized in order to obtain a coating with a 5% atomic substitution of calcium by strontium which corresponds to the best observations on the osteoblast cells activity and on the osteoclast cells proliferation. The physical and chemical characterizations of the obtained coating are carried out by scanning electron microscopy associated to energy dispersive X-ray spectroscopy (EDXS) for X-ray microanalysis and the structural characterization of the coating is carried out by X-ray diffraction. The in vitro dissolution/precipitation properties of the coated substrates are investigated by immersion into Dulbecco's Modified Eagle Medium (DMEM) from 1h to 14 days. The calcium, phosphorus and strontium concentrations variations in the biological liquid are assessed by Induced Coupled Plasma - Atomic Emission Spectroscopy for each immersion time. The results show that under specific experimental conditions, the electrodeposition process is suitable to synthesize strontium-substituted calcium phosphate coatings. Moreover, the addition of hydrogen peroxide (H2O2) into the electrolytic solution used in the process allows us to observe a control of the strontium release during the immersion of the prosthetic materials into DMEM.
锶取代的磷酸钙涂层是通过在钛合金（Ti6Al4V）基底上脉冲电沉积合成的。为了获得钙被锶取代 5%的涂层，优化了该过程的实验条件，这对应于对成骨细胞活性和破骨细胞增殖的最佳观察。通过扫描电子显微镜结合能量色散 X 射线光谱（EDXS）进行 X 射线微分析对获得的涂层进行物理和化学特性分析，以及通过 X 射线衍射对涂层的结构特性进行分析。通过将涂覆的基底浸入 Dulbecco 的改良 Eagle 培养基（DMEM）中 1 小时至 14 天来研究涂覆基底的体外溶解/沉淀特性。通过每个浸入时间的电感耦合等离子体-原子发射光谱法评估生物液中钙、磷和锶浓度的变化。结果表明，在特定的实验条件下，电沉积过程适合合成锶取代的磷酸钙涂层。此外，在该过程中使用的电解液中添加过氧化氢（H2O2），使我们能够观察到在将假体材料浸入 DMEM 中时对锶释放的控制。
