INSERM UMR-S 926, IFR 53, URCA, 21 rue Clément Ader, BP 138, 51685 Reims Cedex 02, France.
J Mater Sci Mater Med. 2011 Apr;22(4):753-61. doi: 10.1007/s10856-011-4251-5. Epub 2011 Feb 3.
Calcium-deficient hydroxyapatite (Ca-def-HAP) coatings on titanium alloy (Ti6Al4V) substrates are elaborated by pulsed electrodeposition. In vitro dissolution/precipitation process is investigated by immersion of the coated substrate into Dulbecco's Modified Eagle Medium (DMEM) from 1 h to 28 days. Calcium and phosphorus concentrations evolution in the biological liquid are determined by Induced Coupled Plasma-Atomic Emission Spectroscopy (ICP-AES) for each immersion time. Physical and chemical characterizations of the coating are performed by scanning electron microscopy (SEM) associated to Energy Dispersive X-ray Spectroscopy (EDXS) for X-ray microanalysis. Surface modifications are investigated by an original method based on the three-dimensional reconstruction of SEM images (3D-SEM). Moreover, corrosion measurements are carried out by potentiodynamic polarization experiments. The results show that the precipitation rate of the Ca-def HAP coating is more pronounced in comparison with that of stoichiometric hydroxyapatite (HAP) used as reference. The precipitated bone-like apatite coating is thick, homogenous and exhibits an improved link to the substrate. Consequently, the corrosion behaviour of the elaborated prosthetic material is improved.
钙缺乏羟基磷灰石 (Ca-def-HAP) 涂层在钛合金 (Ti6Al4V) 基底上通过脉冲电镀进行制备。通过将涂覆的基底浸入到 Dulbecco's Modified Eagle Medium (DMEM) 中 1 小时至 28 天，研究了体外溶解/沉淀过程。通过电感耦合等离子体原子发射光谱法 (ICP-AES) 确定了生物液体中钙和磷浓度的演化，每个浸入时间都进行了测定。通过扫描电子显微镜 (SEM) 结合能量色散 X 射线光谱法 (EDXS) 进行 X 射线微分析，对涂层的物理和化学特性进行了研究。通过基于 SEM 图像的三维重建的原始方法 (3D-SEM) 研究了表面改性。此外，通过动电位极化实验进行了腐蚀测量。结果表明，与用作参考的化学计量羟基磷灰石 (HAP) 相比，Ca-def-HAP 涂层的沉淀速率更为明显。沉淀的类骨磷灰石涂层较厚、均匀，与基底的结合得到改善。因此，所制备的假体材料的腐蚀行为得到了改善。
