Discipline of Marine Biotechnology and Ecology, CSIR - Central Salt and Marine Chemicals Research Institute, G. B. Marg, Bhavnagar 364002, Gujarat, India; Academy of Scientific and Innovative Research, CSIR, New Delhi, India.
Discipline of Marine Biotechnology and Ecology, CSIR - Central Salt and Marine Chemicals Research Institute, G. B. Marg, Bhavnagar 364002, Gujarat, India.
Microbiol Res. 2014 Apr;169(4):301-6. doi: 10.1016/j.micres.2013.07.002. Epub 2013 Jul 31.
Methanol extract of thirty-eight seaweeds samples were first screened against Gram-positive (Staphylococcus aureus ATCC 25923 and Bacillus subtilis ATCC 6051) and -negative (Escherichia coli ATCC 8739 and Pseudomonas aerugenosa ATCC 9027) bacteria. Laurencia papillosa (Ceramiales, Rhodomelaceae, Rhodophyta) gave maximum antimicrobial activity against these bacteria. It was finally tested against four clinical Gram-negative isolates (E. coli, P. aerugenosa, Klebsiella pneumoniae and Shigella flexineri) and exhibited antibacterial activity. The extract was fractionated by column chromatography and the active fraction was identified as a cholesterol derivative, 24-propylidene cholest-5-en-3β-ol using gas chromatography mass spectrometry (GC-MS). The electrospray ionization mass spectrometry (ESI-MS) and FT-IR spectroscopic analysis also supported the structure of the compound. The minimum inhibitory concentration ranged from 1.2 to 1.7 μg/mL (IC50) against clinical isolates. This is the first report of antibacterial activity of this cholesterol derivative. This compound could be exploited as potential lead molecule against broad spectrum drug development. The results also affirm the potential of seaweeds as an important natural source of antimicrobial compounds for pharmaceutical industries.
三十八种海藻样本的甲醇提取物首先针对革兰氏阳性（金黄色葡萄球菌 ATCC 25923 和枯草芽孢杆菌 ATCC 6051）和革兰氏阴性（大肠杆菌 ATCC 8739 和铜绿假单胞菌 ATCC 9027）细菌进行了筛选。角叉菜（红藻门，红毛藻科，红藻）对这些细菌表现出最大的抗菌活性。它最终被测试了对四种临床革兰氏阴性分离株（大肠杆菌、铜绿假单胞菌、肺炎克雷伯菌和福氏志贺菌）的抗菌活性，并表现出了抗菌活性。提取物通过柱色谱法进行分离，活性部分被鉴定为胆固醇衍生物，24-亚甲基胆甾-5-烯-3β-醇，使用气相色谱质谱联用仪（GC-MS）。电喷雾电离质谱（ESI-MS）和傅里叶变换红外光谱分析也支持该化合物的结构。对临床分离株的最小抑菌浓度范围为 1.2 至 1.7 μg/mL（IC50）。这是该胆固醇衍生物抗菌活性的首次报道。该化合物可以作为广谱药物开发的潜在先导分子。该结果也证实了海藻作为制药工业中抗菌化合物的重要天然来源的潜力。
