Tan Marietta, Myers Jeffrey N, Agrawal Nishant
Department of Otolaryngology, Head and Neck Surgery, Johns Hopkins University School of Medicine, 601 North Caroline Street, Baltimore, MD 21287, USA.
Otolaryngol Clin North Am. 2013 Aug;46(4):545-66. doi: 10.1016/j.otc.2013.04.001. Epub 2013 Jun 17.
Recent technological advances now permit the study of the entire cancer genome, which can elucidate complex pathway interactions that are not apparent at the level of single genes. In this review, the authors describe innovations that have allowed for whole-exome/genome analysis of genetic and epigenetic alterations and of changes in gene expression. Studies using next-generation sequencing, array comparative genomic hybridization, methylation arrays, and gene expression profiling are reviewed, with a particular focus on findings from recent whole-exome sequencing projects. A discussion of the implications of these data on treatment and future goals for cancer genomics is included.
近期的技术进步使得对整个癌症基因组的研究成为可能，这能够阐明在单个基因水平上不明显的复杂通路相互作用。在本综述中，作者描述了实现对遗传和表观遗传改变以及基因表达变化进行全外显子组/基因组分析的创新技术。文中回顾了使用新一代测序、阵列比较基因组杂交、甲基化阵列和基因表达谱分析的研究，特别关注了近期全外显子组测序项目的研究结果。还讨论了这些数据对癌症治疗的意义以及癌症基因组学的未来目标。
