Clermont Université, Université Blaise Pascal, Institut Pascal UMR CNRS 6602, 24 Avenue des Landais BP 206, 63174 Aubière cedex, France.
Clermont Université, Université Blaise Pascal, Laboratoire "Microorganismes: Génome et Environnement", UMR CNRS 6023, 24 Avenue des Landais, 63177 Aubière cedex, France.
Bioresour Technol. 2013 Oct;146:732-735. doi: 10.1016/j.biortech.2013.07.030. Epub 2013 Jul 12.
A unicellular Rhodophyte was identified by sequencing of its 18S rRNA encoding gene as belonging to the Rhodella violacea specie. With the objective to optimise the production of biomass and exopolysaccharide by this strain, effects of irradiance, pH and temperature on its photosynthetic activity were investigated. In a second time a stoichiometric study of the well-known f/2 medium led to its supplementation in N and P to increase biomass and then exopolysaccharide yields when the strain was cultivated in photobioreactors. The use of optimal conditions of culture (irradiance of 420 μE/m(2)/s, pH of 8.3 and temperature of 24 °C) and f/2 supplemented medium led to significant increases of biomass and exopolysaccharide productions. The structural characterisation of the produced exopolysaccharide revealed that it was sulphated and mainly composed of xylose. The different culture conditions and culture media tested had no significant impact on the structure of produced exopolysaccharides.
通过对其 18S rRNA 编码基因的测序，鉴定出一种单细胞红藻，属于红藻属。为了优化该菌株的生物量和胞外多糖的生产，研究了光照、pH 值和温度对其光合作用的影响。在第二阶段，对著名的 f/2 培养基进行了化学计量学研究，在光生物反应器中培养该菌株时，补充 N 和 P 以增加生物量，然后增加胞外多糖的产量。使用培养的最佳条件（辐照度为 420 μE/m(2)/s、pH 值为 8.3 和温度为 24°C）和补充 f/2 的培养基，可显著提高生物量和胞外多糖的产量。所产生的胞外多糖的结构特征表明其是硫酸化的，主要由木糖组成。所测试的不同培养条件和培养基对产生的胞外多糖的结构没有显著影响。
