Clermont Auvergne INP, CNRS, Institut Pascal, Université Clermont Auvergne, F-63000 Clermont-Ferrand, France.
Mar Drugs. 2022 Mar 31;20(4):246. doi: 10.3390/md20040246.
Microalgae constitute a remarkable biological diversity but a limited number of them have been the object of study for their ability to produce exoplysaccharides (EPS). Among them, the red marine microalgae or produce sulphated EPS, exhibiting some biological activities with potential interest in the pharmaceutical and cosmetic industries. EPS from and being relatively similar in their composition, it has long been considered that all the red microalgae produced similar EPS and no attention was paid to other red microalgae. The objective of our work was then to explore the diversity of red microalgae for the production of EPS, focusing in this first step on the screening of the strains for their ability to produce EPS and preliminary structural characterization. The study was conducted with 11 microalgae strains belonging to the proteorhodophytina subphylum. All microalgae were able to produce EPS, released in the culture medium (strains belonging to Porphyridiophyceae and Rhodellophyceae classes) or remaining bound to the cells (strains from Stylonematophyceae class). The analysis of monosaccharides composition was found significantly different, with for instance high levels of glucuronic acids in the EPS from and , but also strong differences in the sulphation degrees of polymers (between 1.2 and 28.7% eq. SO).
微藻构成了显著的生物多样性，但只有少数几种因其能够产生胞外多糖 (EPS) 而成为研究对象。其中，红色海洋微藻或产生硫酸化 EPS，具有一些潜在的生物活性，在制药和化妆品行业具有潜在的应用价值。由于 和 的 EPS 在组成上相对相似，长期以来人们认为所有红色微藻产生的 EPS 相似，因此没有关注其他红色微藻。因此，我们的工作目标是探索红色微藻产生 EPS 的多样性，首先集中在筛选具有产生 EPS 能力的菌株和初步结构表征上。本研究使用了属于原甲藻亚门的 11 株微藻菌株。所有微藻都能够产生 EPS，这些 EPS 释放到培养基中（属于藻红藻纲和红藻纲的菌株）或仍然与细胞结合（来自丝状体藻纲的菌株）。单糖组成分析发现差异显著，例如， 和 中的 EPS 中存在高水平的葡萄糖醛酸，但聚合物的硫酸化程度也存在很大差异（1.2 至 28.7%当量 SO）。
