Department of Burns and Cutaneous Surgery, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi, China.
J Surg Res. 2013 Dec;185(2):797-804. doi: 10.1016/j.jss.2013.06.049. Epub 2013 Jul 17.
Severe burns initiate an inflammatory response characterized by the upregulation of proinflammatory cytokine, which contributes to multiple organ injury. Na(+)/H(+) exchanger 1 (NHE1) plays a significant role in several inflammatory processes. This study was designed to investigate the role of NHE1 in burn-induced inflammation and multiple organ injury.
Rats were subjected to a 30% total body surface area full-thickness burn. Cariporide was used to assess the function of NHE1 in burn-induced multiple organ injury by biochemical parameters, histologic changes, and inflammatory cytokine production.
We found that NHE1 expression was significantly increased after burn injury. Inhibition of NHE1 by cariporide attenuated burn-induced edema and tissue injury in heart, lung, kidney, and small intestine. Cariporide also inhibited plasma levels of tumor necrosis factor α, interleukin 6, and myeloperoxidase activity.
These results indicate that NHE1 inhibition prevents burn-induced multiple organ injury. The salutary effects afforded by NHE1 inhibition, at least in part, are mediated by attenuating systemic inflammatory response.
严重烧伤会引发炎症反应，表现为促炎细胞因子的上调，这会导致多器官损伤。钠/氢交换器 1（NHE1）在多种炎症过程中发挥重要作用。本研究旨在探讨 NHE1 在烧伤诱导的炎症和多器官损伤中的作用。
大鼠接受 30%总体表面积全层烧伤。用 cariporide 通过生化参数、组织学变化和炎性细胞因子产生来评估 NHE1 在烧伤诱导的多器官损伤中的作用。
我们发现 NHE1 表达在烧伤后显著增加。用 cariporide 抑制 NHE1 可减轻烧伤引起的心脏、肺、肾和小肠水肿和组织损伤。Cariporide 还抑制了肿瘤坏死因子-α、白细胞介素 6 和髓过氧化物酶活性的血浆水平。
这些结果表明，NHE1 抑制可预防烧伤诱导的多器官损伤。NHE1 抑制的有益作用至少部分是通过减轻全身炎症反应来实现的。
