Department of Analytical Chemistry, Marie Curie Building (Annex), Campus de Rabanales, University of Córdoba, E-14071 Córdoba, Spain.
Anal Chim Acta. 2013 Aug 20;792:93-100. doi: 10.1016/j.aca.2013.07.004. Epub 2013 Jul 9.
Here, a creatinine-modified CdSe/ZnS quantum dots fluorescent probe has been prepared and used for sensing 2,4,6,-trinitrotoluene explosive (TNT). The proposed method is based on the selective interaction between creatinine and nitroaromatic compounds according to the well-known Jaffé reaction. The procedure for the synthesis of creatinine-CdSe/ZnS reagent is very simple and reproducible and its fluorescent characteristics are reported. We found that the presence of TNT quenches the original fluorescence of creatinine-QD according to the Stern-Volmer model. Under the working conditions, the calibration plot of Io/I versus concentration of TNT was linear in the range 10-300μg L(-1) (R(2)=0.996). The mechanism interaction is discussed. The selectivity of fluorescence quenching of creatinine-QD for TNT has been evaluated. Finally, the potential application of the proposed methodology for the determination of TNT in spiked soils is demonstrated. For the analysis of soil samples a solid-liquid extraction is carried out and a four-point standard addition protocol is used to correct the matrix effect. The method, which is simple and rapid, allows the detection of 0.057μg g(-1) of TNT in soil samples. This sensor could be a useful tool for environmental studies, a crucial topic for nanotechnology nowadays.
这里，我们制备了一种基于肌酸改性的 CdSe/ZnS 量子点荧光探针，并将其用于检测 2,4,6-三硝基甲苯（TNT）爆炸物。该方法是基于肌酸与硝基芳香族化合物之间的选择性相互作用，根据著名的 Jaffé 反应原理。肌酸-CdSe/ZnS 试剂的合成过程非常简单、可重现，且其荧光特性也有相关报道。我们发现，TNT 的存在根据 Stern-Volmer 模型猝灭了肌酸-QD 的原始荧光。在工作条件下，Io/I 与 TNT 浓度的校准曲线在 10-300μg L(-1) 范围内呈线性（R(2)=0.996）。此外，我们还对相互作用机制进行了讨论。肌酸-QD 对 TNT 的荧光猝灭具有选择性。最后，我们还展示了该方法在加标土壤中 TNT 测定方面的潜在应用。对于土壤样品的分析，我们进行了固液萃取，并用四点标准加入法校正基体效应。该方法简单、快速，允许检测土壤样品中 0.057μg g(-1) 的 TNT。该传感器有望成为环境研究领域的一种有用工具，这也是当今纳米技术的一个关键课题。
