The Petroleum and Petrochemical College, Chulalongkorn University, Soi Chula 12, Phyathai Road, Pathumwan, Bangkok 10330, Thailand.
Carbohydr Polym. 2013 Sep 12;97(2):441-50. doi: 10.1016/j.carbpol.2013.04.076. Epub 2013 May 3.
Coupling of chitin whisker (CTWK) or water-soluble chitosan (WSCS) with magnetic nanoparticles (MAG) via a covalent linkage by using a silane coupling agent in an aqueous system is proposed. The use of the silane coupling agent glycidoxypropyltrimethoxy, having a trimethoxy silane as well as an epoxide functionality, allows one step coupling between CTWK or WSCS and MAG. The MAG derivatives obtained are well dispersed and their colloidal aqueous solutions can be stabilized for days. An external magnetic field initiates alignment of nano-rod structured CTWK-MAG in the direction of the magnetic field. The uniqueness of these two materials is that CTWK-MAG shows solvent polarity responsiveness whereas WSCS-MAG performs pH responsiveness. A model application study on DNA extraction confirms that WSCS-MAG with positively charged at pH 7 and with high surface area performs more Escherichia coli extraction ability for two times as compared to CTWK-MAG.
通过在水相体系中使用硅烷偶联剂，将壳聚糖纳米纤维（CTWK）或水溶性壳聚糖（WSCS）与磁性纳米颗粒（MAG）通过共价键连接起来。使用硅烷偶联剂缩水甘油丙基三甲氧基，它具有三甲氧基硅烷和环氧化物官能团，可以在 CTWK 或 WSCS 和 MAG 之间进行一步偶联。得到的 MAG 衍生物分散良好，其胶体水溶液可以稳定数天。外加磁场引发纳米棒结构的 CTWK-MAG 在磁场方向上的对齐。这两种材料的独特之处在于，CTWK-MAG 表现出溶剂极性响应性，而 WSCS-MAG 则表现出 pH 响应性。在 DNA 提取的模型应用研究中证实，在 pH 值为 7 时带正电荷且具有高表面积的 WSCS-MAG 对大肠杆菌的提取能力是 CTWK-MAG 的两倍。
