School of Life Science and National Glycoengineering Research Center, Shandong University, Jinan 250100, China.
Carbohydr Polym. 2013 Sep 12;97(2):800-8. doi: 10.1016/j.carbpol.2013.05.068. Epub 2013 Jun 1.
In this study, we reported that polysaccharides (RPS) from the mycelia of liquid-cultured Rhizopus nigricans inhibited the proliferation and clonogenic potential of human gastric cancer BGC-823 cells. Results of acridine orange/ethidium bromide and Hoechst 33258 staining showed that treated cells displayed typical morphological characteristics of apoptosis such as condensation of chromatin, nuclear pyknosis and formation of apoptotic bodies. Flow cytometry analyses and colorimetric assay indicated that RPS induced mitochondria-mediated apoptosis which was associated with collapse of mitochondrial membrane potential, activation of caspase-9 and caspase-3, generation of intracellular reactive oxygen species and elevation of intracellular calcium in BGC-823 cells. Moreover, cell-cycle analysis revealed that RPS arrested cells in the G2/M phase of the cell cycle. These results provided further insights into the potential use of RPS as an anti-cancer agent against human gastric cancer.
在这项研究中，我们报道了从液体培养的黑根霉菌丝体中提取的多糖（RPS）抑制了人胃癌 BGC-823 细胞的增殖和克隆形成能力。吖啶橙/溴化乙锭和 Hoechst 33258 染色的结果表明，处理后的细胞显示出典型的凋亡形态特征，如染色质凝聚、核固缩和凋亡小体形成。流式细胞术分析和比色法分析表明，RPS 诱导了线粒体介导的凋亡，这与线粒体膜电位崩溃、半胱天冬酶-9 和半胱天冬酶-3 的激活、细胞内活性氧的产生以及 BGC-823 细胞内钙离子的升高有关。此外，细胞周期分析显示，RPS 将细胞阻滞在细胞周期的 G2/M 期。这些结果为 RPS 作为一种抗人类胃癌的抗癌剂的潜在用途提供了进一步的见解。
