Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, Dalian 116023, China.
Fish Shellfish Immunol. 2013 Nov;35(5):1654-7. doi: 10.1016/j.fsi.2013.07.020. Epub 2013 Aug 2.
The sea cucumber, Apostichopus japonicus possesses a variety of cells populating in both the coelomic (cells in the coelomic are called coelomocytes) and water-vascular system. In this study, we compared cells in these two systems of A. japonicus on total cell number, cell types and surface antigens through monoclonal antibodies against coelomocytes. The results demonstrated that the cell types observed in coelomic also could be found in water-vascular system, but the total cell number and percentages of each type were different. The total number of coelomocytes was 2-3 times of that in water-vascular system. Lymphoid cells were numerically dominant in coelomic system, while spherulocytes with pseudopods in water-vascular system. Results of indirect immunofluorescence assay technique showed that both coelomocytes and cells in water-vascular system could be recognized by the corresponding MAbs, and the distribution of its positive signals was not different. In conclusion, cell types and surface antigens in coelomic and water-vascular system were same, but the total cell number and percentages of each type were different. And further researches are needed on whether there are differences in functions of the different composition.
海参，刺参具有多种细胞在体腔（细胞在体腔中被称为体腔细胞）和水脉系统中分布。在这项研究中，我们通过针对体腔细胞的单克隆抗体比较了 A. japonicus 这两个系统中的细胞在总细胞数、细胞类型和表面抗原方面的差异。结果表明，在体腔中观察到的细胞类型也可以在水脉系统中找到，但总细胞数和每种类型的百分比不同。体腔细胞的总数是水脉系统的 2-3 倍。淋巴样细胞在体腔系统中数量上占优势，而水脉系统中的球形细胞具有伪足。间接免疫荧光检测技术的结果表明，体腔细胞和水脉系统中的细胞都可以被相应的单克隆抗体识别，其阳性信号的分布没有差异。总之，体腔和水脉系统中的细胞类型和表面抗原相同，但每种类型的总细胞数和百分比不同。需要进一步研究不同组成部分的功能是否存在差异。
