Li Qiang, Qi Rui-rong, Wang Yi-nan, Qiao Guo, Ye Shi-gen, Li Hua
Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, Dalian 116023, China.
Department of Ocean Technology, College of Chemistry and Biology Engineering, Yancheng Institute of Technology, Yancheng 224051, China.
Fish Shellfish Immunol. 2014 Dec;41(2):260-3. doi: 10.1016/j.fsi.2014.09.005. Epub 2014 Sep 16.
Monoclonal antibodies (MAbs) specifically against coelomocytes of Apostichopus japonicus were employed to study the ontogenesis of coelomocytes by indirect immunofluorescence assay technique (IIFAT). Different developmental stages were identified by histochemical staining method. Stages including blastula, gastrula, auricularia (small-auricular larvae, mid-auricular larvae and big-auricular larvae), doliolaria, pentactula and juvenile were examined. The positive reactions with both MAb1C2 against all the types of coelomocytes and MAb3F6 specific to spherulocytes, were observed firstly at the blastula stage of the embryos. The positive reaction with MAb1E2 against lymphoid cells was observed from the big-auricular larvae, which indicated that lymphoid cells may not be progenitor cells or stem cells for A. japonicus. An increase of fluorescence intensity for each cell may imply a possible enhancement of the innate defensive mechanism as the embryogenesis progress.
采用特异性针对刺参体腔细胞的单克隆抗体，通过间接免疫荧光分析技术（IIFAT）研究体腔细胞的个体发生。通过组织化学染色方法鉴定不同发育阶段。检查了包括囊胚、原肠胚、耳状幼体（小耳状幼虫、中耳状幼虫和大耳状幼虫）、桶状幼虫、五触手幼体和幼参在内的各个阶段。针对所有类型体腔细胞的单克隆抗体1C2以及特异性针对球形细胞的单克隆抗体3F6的阳性反应，首先在胚胎的囊胚阶段观察到。针对淋巴细胞的单克隆抗体1E2的阳性反应从大耳状幼虫期开始观察到，这表明淋巴细胞可能不是刺参的祖细胞或干细胞。随着胚胎发育的进行，每个细胞荧光强度的增加可能意味着先天防御机制可能得到增强。
