Department of Urology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China.
Mol Med Rep. 2013 Oct;8(4):999-1004. doi: 10.3892/mmr.2013.1612. Epub 2013 Aug 2.
The aim of the present study was to investigate the functions of RACK1 and its involvement in mechanisms of prostate cancer (PC) cell proliferation, invasion and metastasis. The proliferation, invasion and metastasis of stably transfected DU145 cells with RACK1 was evaluated in vitro as well as in vivo following the establishment of nude mouse models. The expression of Ki67, RACK1, PTEN and androgen receptor (AR) in PC was detected by immunohistochemical analysis. Our results indicated that RACK1 promotes PC cell proliferation, invasion and metastasis in vitro and in vivo. However, knockdown of RACK1 by siRNA in vitro inhibited PC cell proliferation, migration and invasion. PTEN downregulation and Ki67 upregulation were also altered with the upregulation of RACK1; RACK1 staining was strongly correlated with PTEN downregulation and Ki67 upregulation. These data demonstrated that increased RACK1 expression is important in promoting PC cell proliferation, invasion and metastasis in vitro and in vivo.
本研究旨在探究 RACK1 的功能及其在前列腺癌（PC）细胞增殖、侵袭和转移机制中的作用。通过建立裸鼠模型，在体外和体内评估稳定转染 RACK1 的 DU145 细胞的增殖、侵袭和转移。采用免疫组化分析检测 PC 中 Ki67、RACK1、PTEN 和雄激素受体（AR）的表达。结果表明，RACK1 促进 PC 细胞在体外和体内的增殖、侵袭和转移。然而，体外 siRNA 敲低 RACK1 抑制了 PC 细胞的增殖、迁移和侵袭。PTEN 下调和 Ki67 上调也随 RACK1 的上调而改变；RACK1 染色与 PTEN 下调和 Ki67 上调呈强相关性。这些数据表明，RACK1 表达增加在促进 PC 细胞的增殖、侵袭和转移中起着重要作用，无论是在体外还是在体内。
