Shen Congcong, Hua Hui, Gu Lixiong, Cao Shuanglin, Cai Hengji, Yao Xiaodong, Chen Xiaodong
Department of Dermatology, Affiliated Hospital of Nantong University, Nantong, 226001, P.R. China.
Department of Dermatology, The Third People's Hospital of Nantong, Nantong, 226001, P.R. China.
J Cancer. 2020 Jan 1;11(4):795-803. doi: 10.7150/jca.36905. eCollection 2020.
Melanoma is a highly malignant skin cancer with limited treatment options, the mechanism of the occurrence and development of melanoma is still unclear till now. Receptor for activated C kinase 1 (RACK1) is a scaffolding protein that mediates multiple signaling pathways; it interconnects distinct signaling pathways to control essential cellular processes. RACK1 was reported as an oncogene in human tumorigenesis, but little is known about its role in melanoma. This study aimed to investigate the expression of RACK1 in patients with melanoma and to reveal its possible functions in melanoma cells. The expression profiles of RACK1 detected in tumor tissues from melanoma patients showed that RACK1 was higher in tumor tissues, and its expression level was well associated with the clinical progression of melanoma (TNM stage, P=0.009). Furthermore, RNA interfering (RNAi) knockdown of RACK1 could efficiently suppress the proliferation, migration and invasion of A375 and A875 cells and promote their apoptosis. Taken together, these results suggest that RACK1 may be a poor prognostic factor in human melanoma, and it may be a new therapeutic target for melanoma treatment.
黑色素瘤是一种恶性程度很高的皮肤癌，治疗选择有限，迄今为止，黑色素瘤发生和发展的机制仍不清楚。活化C激酶1受体（RACK1）是一种介导多种信号通路的支架蛋白；它将不同的信号通路相互连接以控制重要的细胞过程。RACK1在人类肿瘤发生过程中被报道为一种癌基因，但对其在黑色素瘤中的作用知之甚少。本研究旨在调查RACK1在黑色素瘤患者中的表达情况，并揭示其在黑色素瘤细胞中的可能功能。在黑色素瘤患者肿瘤组织中检测到的RACK1表达谱表明，RACK1在肿瘤组织中表达较高，其表达水平与黑色素瘤的临床进展（TNM分期，P=0.009）密切相关。此外，RNA干扰（RNAi）敲低RACK1可有效抑制A375和A875细胞的增殖、迁移和侵袭，并促进其凋亡。综上所述，这些结果表明RACK1可能是人类黑色素瘤预后不良的一个因素，并且可能是黑色素瘤治疗的一个新的治疗靶点。
