CAS Key Laboratory of Computational Biology, CAS-MPG (Chinese Academy of Sciences-German Max Planck Society) Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, China.
Photosynth Res. 2013 Nov;117(1-3):339-54. doi: 10.1007/s11120-013-9894-2. Epub 2013 Aug 3.
Chlorophyll a fluorescence induction (FI) kinetics, in the microseconds to the second range, reflects the overall performance of the photosynthetic apparatus. In this paper, we have developed a novel FI model, using a rule-based kinetic Monte Carlo method, which incorporates not only structural and kinetic information on PSII, but also a simplified photosystem I. This model has allowed us to successfully simulate the FI under normal or different treatment conditions, i.e., with different levels of measuring light, under 3-(3',4'-dichlorophenyl)-1,1-dimethylurea treatment, under 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone treatment, and under methyl viologen treatment. Further, using this model, we have systematically studied the mechanistic basis and factors influencing the FI kinetics. The results of our simulations suggest that (1) the J step is caused by the two-electron gate at the Q B site; (2) the I step is caused by the rate limitation of the plastoquinol re-oxidation in the plastoquinone pool. This new model provides a framework for exploring impacts of modifying not only kinetic but also structural parameters on the FI kinetics.
叶绿素 a 荧光诱导（FI）动力学在微秒到秒的范围内反映了光合作用装置的整体性能。在本文中，我们使用基于规则的动力学蒙特卡罗方法开发了一种新的 FI 模型，该模型不仅结合了 PSII 的结构和动力学信息，还简化了光合作用系统 I。该模型使我们能够成功模拟正常或不同处理条件下的 FI，即在不同测量光水平下、在 3-（3'，4'-二氯苯基）-1,1-二甲基脲处理下、在 2,5-二溴-3-甲基-6-异丙基-p-苯醌处理下以及在甲紫精处理下。此外，我们使用该模型系统地研究了 FI 动力学的机制基础和影响因素。模拟结果表明：（1）J 步是由 QB 位点的双电子门引起的；（2）I 步是由质体醌库中质体醌还原的速率限制引起的。这个新模型为探索不仅改变动力学而且改变结构参数对 FI 动力学的影响提供了一个框架。
